Document Detail


Aleurone cell walls of wheat grain: high spatial resolution investigation using synchrotron infrared microspectroscopy.
MedLine Citation:
PMID:  18702863     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infrared microspectroscopy and immunolabeling techniques were employed in order to obtain deeper insight into the biochemical nature of aleurone cell walls of wheat grain. The use of a synchrotron source, thanks to its intrinsic brightness, has provided unprecedented information at the level of a few micrometers and has allowed the discrimination of various polysaccharides in cell walls. The high spectral quality obtained in the small analyzed domain has been beneficial in estimating the relative proportions of beta-glucan and arabinoxylan, through the use of principal component analysis (PCA). The highest amount of beta-glucan is found in periclinal cell walls close to the starchy endosperm. The junction regions between aleurone cells are enriched in arabinoxylan. At the early stage of wheat grain development (271 degrees D), the chemical composition along the cell walls is more heterogeneous than at the mature stage. Both synchrotron infrared microspectroscopy and immunolabeling experiments made it possible to reveal the spatial heterogeneity of the various chemical compositions of aleurone cell walls.
Authors:
F Jamme; P Robert; B Bouchet; L Saulnier; P Dumas; F Guillon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied spectroscopy     Volume:  62     ISSN:  0003-7028     ISO Abbreviation:  Appl Spectrosc     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-15     Completed Date:  2008-09-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372406     Medline TA:  Appl Spectrosc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  895-900     Citation Subset:  IM    
Affiliation:
Synchrotron SOLEIL, L'Orme des Merisiers, BP 48, Saint Aubin, F-91192, Gif-sur-yvette, France. frederic.jamme@synchrotron-soleil.fr
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MeSH Terms
Descriptor/Qualifier:
Cell Wall / chemistry*
Plant Extracts / chemistry
Plant Proteins / chemistry*
Spectroscopy, Fourier Transform Infrared / methods
Synchrotrons
Triticum* / chemistry,  cytology
Xylans / chemistry
beta-Glucans / chemistry
Chemical
Reg. No./Substance:
0/Plant Extracts; 0/Plant Proteins; 0/Xylans; 0/beta-Glucans; 9040-27-1/arabinoxylan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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