| Aleurone cell walls of wheat grain: high spatial resolution investigation using synchrotron infrared microspectroscopy. | |
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MedLine Citation:
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PMID: 18702863 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Infrared microspectroscopy and immunolabeling techniques were employed in order to obtain deeper insight into the biochemical nature of aleurone cell walls of wheat grain. The use of a synchrotron source, thanks to its intrinsic brightness, has provided unprecedented information at the level of a few micrometers and has allowed the discrimination of various polysaccharides in cell walls. The high spectral quality obtained in the small analyzed domain has been beneficial in estimating the relative proportions of beta-glucan and arabinoxylan, through the use of principal component analysis (PCA). The highest amount of beta-glucan is found in periclinal cell walls close to the starchy endosperm. The junction regions between aleurone cells are enriched in arabinoxylan. At the early stage of wheat grain development (271 degrees D), the chemical composition along the cell walls is more heterogeneous than at the mature stage. Both synchrotron infrared microspectroscopy and immunolabeling experiments made it possible to reveal the spatial heterogeneity of the various chemical compositions of aleurone cell walls. |
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Authors:
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F Jamme; P Robert; B Bouchet; L Saulnier; P Dumas; F Guillon |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied spectroscopy Volume: 62 ISSN: 0003-7028 ISO Abbreviation: Appl Spectrosc Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-15 Completed Date: 2008-09-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372406 Medline TA: Appl Spectrosc Country: United States |
Other Details:
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Languages: eng Pagination: 895-900 Citation Subset: IM |
Affiliation:
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Synchrotron SOLEIL, L'Orme des Merisiers, BP 48, Saint Aubin, F-91192, Gif-sur-yvette, France. frederic.jamme@synchrotron-soleil.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Wall
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chemistry* Plant Extracts / chemistry Plant Proteins / chemistry* Spectroscopy, Fourier Transform Infrared / methods Synchrotrons Triticum* / chemistry, cytology Xylans / chemistry beta-Glucans / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Plant Extracts; 0/Plant Proteins; 0/Xylans; 0/beta-Glucans; 9040-27-1/arabinoxylan |
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