Document Detail

Aldosterone action: induction of p21(ras) and fra-2 and transcription-independent decrease in myc, jun, and fos.
MedLine Citation:
PMID:  10329965     Owner:  NLM     Status:  MEDLINE    
Adrenal steroids induce an increase in transcellular Na+ reabsorption across Xenopus laevis A6 cell epithelia that requires the action of transcriptionally regulated gene products. In a previous study we identified K-ras2 as an aldosterone-upregulated mRNA in A6 epithelia. Here, we show that in vivo injection of aldosterone in Xenopus (2.5 h) increases K-ras2 mRNA specifically in the kidney (2.5-fold) and that in A6 epithelia aldosterone (2.5 h) increases Ras protein synthesis ( approximately 6-fold). Xl-ras, another ras mRNA expressed at a low level in A6 cells, was also induced (2-fold). Aldosterone was shown to regulate the mRNA levels of several transcription factors as well. After 2 h of aldosterone treatment, fra-2 mRNA was upregulated by 130%, whereas c-myc, c-jun, c-fos, and glucocorticoid receptor mRNAs were downregulated by 23-43%. After 16 h, c-fos and GR mRNAs were further decreased, whereas levels of fra-2, c-jun, and c-myc began to return to control levels. Interestingly, the downregulation of the protooncogene mRNAs was independent of transcription. These results support the view that aldosterone exerts complex pleiotropic transcriptional and nontranscriptional actions that involve the regulation of signaling cascade elements (i.e., K-Ras2) as well as that of transcription factors.
B Spindler; F Verrey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  276     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-06-07     Completed Date:  1999-06-07     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C1154-61     Citation Subset:  IM    
Institute of Physiology, University of Zürich, CH-8057 Zürich, Switzerland.
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MeSH Terms
Aldosterone / pharmacology*
Blotting, Northern
Cell Line
Cloning, Molecular
DNA-Binding Proteins / genetics*
Epithelial Cells / metabolism
Fos-Related Antigen-2
Gene Expression / drug effects
Genes, fos / genetics*
Genes, jun / genetics*
Genes, myc / genetics*
Genes, ras / genetics*
Kidney / metabolism
Polymerase Chain Reaction
RNA, Messenger / biosynthesis
Receptors, Glucocorticoid / genetics
Sodium-Potassium-Exchanging ATPase / genetics
Transcription Factors / genetics*
Xenopus laevis
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Fos-Related Antigen-2; 0/RNA, Messenger; 0/Receptors, Glucocorticoid; 0/Transcription Factors; 52-39-1/Aldosterone; EC ATPase

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