| Aldehyde modification of peptide immunogen enhances protein-reactive antibody response to toxic shock syndrome toxin-1. | |
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MedLine Citation:
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PMID: 17131300 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Introduction of aldehyde groups into protein conjugates enhanced the immune response to a coupled peptide without the use of strong adjuvants. Synthetic peptides representing the N-terminal (residues 1-16) and internal (residues 53-65) epitopes of toxic shock syndrome toxin-1 (TSST-1) were coupled to carrier protein, and carbonyl tags were introduced by Amadori reaction with glycolaldehyde. Modified and unmodified antigens in alum were used to immunize rabbits and the reactivities of antisera were compared. Aldehyde modification augmented the response detected by ELISA, which included enhanced binding to peptides and to native TSST-1. In western blot, TSST-1 was detected by antiserum elicited to the N-terminal peptide, but not that generated to the peptide representing the internal sequence. The same antiserum also neutralized TSST-1 activity in a lymphocyte proliferation assay. The circular dichroism spectrum of the N-terminal peptide indicated a propensity for helical conformation, similar to the structure at the corresponding sequence of the native protein. These data suggest that aldehyde modification can boost immunogenicity of peptide-based vaccines, generating epitope-specific immune responses against the cognate protein antigens without using potent adjuvants. |
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Authors:
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Alfonso Bavoso; Angela Ostuni; Jolanda De Vendel; Francesco Pollaro; Francesca Armentano; Thomas Knight; Sudesh Makker; Alfonso Tramontano |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of peptide science : an official publication of the European Peptide Society Volume: 12 ISSN: 1075-2617 ISO Abbreviation: J. Pept. Sci. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-12-04 Completed Date: 2007-01-25 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 9506309 Medline TA: J Pept Sci Country: England |
Other Details:
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Languages: eng Pagination: 843-9 Citation Subset: IM |
Affiliation:
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Universita' degli Studi della Basilicata, Department of Chemistry, Potenza, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aldehydes
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chemistry Amino Acid Sequence Animals Bacterial Toxins / immunology, toxicity Blotting, Western Cell Proliferation / drug effects Circular Dichroism Enzyme-Linked Immunosorbent Assay Epitopes / chemistry, immunology Immune Sera / immunology Lymphocytes / cytology, drug effects Mice Mice, Inbred BALB C Molecular Sequence Data Peptides / chemical synthesis*, chemistry, immunology Rabbits Staphylococcus aureus / immunology |
| Grant Support | |
ID/Acronym/Agency:
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CA90564/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aldehydes; 0/Bacterial Toxins; 0/Epitopes; 0/Immune Sera; 0/Peptides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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