| Aldehyde dehydrogenase heterogeneity in rat hepatic cells. | |
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MedLine Citation:
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PMID: 2310196 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In normal rat liver, aldehyde dehydrogenase (Aldehyde:NAD+ oxidoreductase, EC 1.2.1.3; ALDH) is found primarily in mitochondrial and microsomal fractions. During hepatocarcinogenesis, an additional tumor-associated aldehyde dehydrogenase (T-ALDH) is detectable in the cytosol of preneoplastic and neoplastic cells. We report here differences in the ALDH distribution pattern in different rat hepatoma cell lines compared to normal rat hepatocytes. Of the four basal ALDH enzymes, one mitochondrial ALDH and one microsomal ALDH account for 96% of total ALDH molecules detectable with our probes in normal hepatocytes. The other two mitochondrial and microsomal ALDH enzymes are only detectable in the appropriate subcellular fraction from large populations of cells. The tumor-associated ALDH is not detectable in normal hepatocytes. In addition to varying amounts of T-ALDH in the six different rat hepatoma cell lines examined, differences in the amounts of mitochondrial and microsomal ALDHs also occur in both high and low T-ALDH activity hepatoma cell lines. Each of five ALDH enzymes examined has a characteristic half-life varying from 45 min to 95 h. |
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Authors:
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M Huang; R Lindahl |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 277 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1990 Mar |
Date Detail:
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Created Date: 1990-04-06 Completed Date: 1990-04-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 296-300 Citation Subset: IM |
Affiliation:
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Department of Biology, University of Alabama, Tuscaloosa 35486. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aldehyde Dehydrogenase
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isolation & purification,
metabolism* Animals Cells, Cultured Isoenzymes / isolation & purification, metabolism* Kinetics Liver / enzymology* Liver Neoplasms, Experimental / enzymology* Microsomes, Liver / enzymology Mitochondria, Liver / enzymology Rats |
| Grant Support | |
ID/Acronym/Agency:
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CA-21103/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; EC 1.2.1.3/Aldehyde Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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