Document Detail

Aldehyde dehydrogenase-dependent acetaldehyde metabolism in periportal and pericentral regions of the perfused rat liver.
MedLine Citation:
PMID:  6827478     Owner:  NLM     Status:  MEDLINE    
NADH fluorescence (366 leads to 450 nm) was measured with a large tipped light guide from the surface of rat liver perfused with hemoglobin-free medium without recirculation. A good correlation (r = 0.93) was found between the increase in NADH fluorescence and the rate of oxidation of infused acetaldehyde (0.1-4.0 mM). By using microlight guides placed on either periportal or pericentral areas of the liver, it was found that infusion of acetaldehyde increased NADH fluorescence first in periportal regions and then in pericentral areas. During retrograde perfusions, NADH fluorescence in pericentral areas increased before portal areas. By using the correlation between the increase in NADH fluorescence and the rate of acetaldehyde uptake established with the large light guide, rates of acetaldehyde uptake in pericentral and periportal areas were estimated from the microlight guide measurement of the periportal and pericentral NADH fluorescence increments caused by acetaldehyde infusions. Half-maximal acetaldehyde uptake was observed at about 0.8 mM in both regions. Maximal rates of acetaldehyde uptake were 212 and 197 mumol/g/hr in periportal and pericentral regions, respectively. The results indicate that rates of aldehyde dehydrogenase-dependent acetaldehyde oxidation are similar in periportal and pericentral regions of the liver lobule.
T Kashiwagi; K O Lindros; S Ji; R G Thurman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of pharmacology and experimental therapeutics     Volume:  224     ISSN:  0022-3565     ISO Abbreviation:  J. Pharmacol. Exp. Ther.     Publication Date:  1983 Mar 
Date Detail:
Created Date:  1983-04-07     Completed Date:  1983-04-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376362     Medline TA:  J Pharmacol Exp Ther     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  538-42     Citation Subset:  IM    
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MeSH Terms
Acetaldehyde / metabolism*
Aldehyde Dehydrogenase
Aldehyde Oxidoreductases / metabolism*
Liver / enzymology*
NAD / metabolism
Portal System
Pyrazoles / pharmacology
Rats, Inbred Strains
Grant Support
Reg. No./Substance:
0/Pyrazoles; 53-84-9/NAD; 75-07-0/Acetaldehyde; 7554-65-6/fomepizole; EC 1.2.-/Aldehyde Oxidoreductases; EC Dehydrogenase

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