Document Detail


Alcohol dehydrogenase and catalase content in perinatal infant and adult livers: potential influence on neonatal alcohol metabolism.
MedLine Citation:
PMID:  17343998     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Methanol and ethanol are primarily metabolized through the alcohol dehydrogenase (ADH) system in adults. Under saturating substrate concentrations, blood alcohol concentrations decline at a constant rate (i.e., zero-order kinetics). Minor metabolic alcohol pathways include oxidation through the cytochrome P450 system and catalase. We previously reported a 5-week-old infant that survived intoxication at an exceptional methanol concentration (1148 mg/dL). As the rate of elimination followed first-order kinetics, we proposed that an alternate non-saturating alcohol metabolizing enzyme system such as catalase was responsible for this observation. In this study, we developed and optimized enzyme immunoassays for tissue ADH and catalase, and measured the concentration of these enzymes in the livers of 18 adults and 7 perinatal infants obtained from autopsies. The mean ADH content of perinatal infants was approximately 10-fold lower than adults (0.11+/-0.09 g/kg versus 1.00+/-0.37 g/kg liver wet weight, respectively). The alphaalpha isoenzyme of ADH was the predominant isoform in perinatal infants, while there were several isoenzymes found in adult livers. For catalase, half of the perinatal infants (n=4) had roughly equal amounts as adults (0.32+/-0.03 g/kg versus 0.37+/-0.20 g/kg wet weight, p=NS), while three other perinatal infants had significantly higher concentrations (1.55+/-0.48 g/kg versus 0.37+/-0.02 g/kg wet weight, p<0.05). In the perinatal infants, these results suggest that catalase may play a more prominent role in the metabolism of alcohols than does the ADH system and may explain the finding of first-order kinetics in case reports of high methanol and ethanol intoxication.
Authors:
Minh-Nguyet Tran; Alan H B Wu; Dennis W Hill
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Publication Detail:
Type:  Journal Article     Date:  2007-02-06
Journal Detail:
Title:  Toxicology letters     Volume:  169     ISSN:  0378-4274     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-26     Completed Date:  2007-05-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  245-52     Citation Subset:  IM    
Affiliation:
Department of Pathobiology, University of Connecticut, Storrs, CT, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Aging / metabolism*
Alcohol Dehydrogenase / metabolism*
Alcohols / metabolism*,  pharmacokinetics
Catalase / metabolism*
Female
Humans
Infant
Infant, Newborn
Isoenzymes / metabolism
Liver / enzymology*,  growth & development
Male
Middle Aged
Chemical
Reg. No./Substance:
0/Alcohols; 0/Isoenzymes; EC 1.1.1.1/Alcohol Dehydrogenase; EC 1.11.1.6/Catalase

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