Document Detail


Alanine enhances jejunal sodium absorption in the presence of glucose: studies in piglet viral diarrhea.
MedLine Citation:
PMID:  3018659     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We measured the response of jejunal sodium (Na) absorption to neutral amino acid (L-alanine) and to dipeptides (L-alanyl-L-alanine, glycylsarcosine) in normal piglets and in piglets with acute viral diarrhea after experimental infection with transmissible gastroenteritis (TGE) virus. In the TGE jejunum villi were blunted, crypts were deepened, and the epithelium was composed of relatively undifferentiated cells with reduced disaccharidase, decreased sodium-potassium-stimulated ATPase, and elevated thymidine kinase activities. The response of Na absorption to a maximal concentration of L-alanine (20 mM) or D-glucose (30 mM) was significantly blunted in TGE jejunum in Ussing chambers. However, the addition of L-alanine together with D-glucose caused a significantly greater increment of Na absorption than either L-alanine or D-glucose alone in control and TGE tissue. The effect of Na absorption of the dipeptide L-alanyl-L-alanine (10 mM), which was rapidly hydrolyzed by control and TGE mucosa, was similar to that of L-alanine (20 mM), while glycylsarcosine, a poorly hydrolyzed dipeptide, did not change net Na absorption in the jejunum. Our data support the concept of separate carrier systems for neutral amino acid and hexose in the crypt-type intestinal epithelium characterizing viral enteritis. We speculate that a sodium-cotransporting amino acid, if added to oral glucose-electrolyte solutions, could benefit oral rehydration therapy in acute viral diarrhea; neither of the dipeptides tested here can be expected to enhance absorption to any greater extent than its constituent amino acids.
Authors:
J M Rhoads; R J MacLeod; J R Hamilton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pediatric research     Volume:  20     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  1986 Sep 
Date Detail:
Created Date:  1986-10-22     Completed Date:  1986-10-22     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  879-83     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alanine / pharmacology*
Animals
Gastroenteritis, Transmissible, of Swine / metabolism*
Glucose / metabolism*
Intestinal Absorption / drug effects*
Intestinal Mucosa / drug effects,  metabolism
Jejunum / drug effects,  metabolism*
Kinetics
Lactose / metabolism
Reference Values
Sodium / metabolism*
Sodium-Potassium-Exchanging ATPase / metabolism
Sucrase / metabolism
Swine
Thymidine Kinase / metabolism
Chemical
Reg. No./Substance:
50-99-7/Glucose; 56-41-7/Alanine; 63-42-3/Lactose; 7440-23-5/Sodium; EC 2.7.1.21/Thymidine Kinase; EC 3.2.1.48/Sucrase; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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