| Airway hyperresponsiveness in allergically inflamed mice: the role of airway closure. | |
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MedLine Citation:
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PMID: 17255559 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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RATIONALE: Allergically inflamed mice exhibit airway hyperresponsiveness to inhaled methacholine, which computer simulations of lung impedance suggest is due to enhanced lung derecruitment and which we sought to verify in the present study. METHODS: BALB/c mice were sensitized and challenged with ovalbumin to induce allergic inflammation; the control mice were sensitized but received no challenge. The mice were then challenged with inhaled methacholine and respiratory system impedance tracked for the following 10 minutes. Respiratory elastance (H) was estimated from each impedance measurement. One group of mice was ventilated with 100% O(2) during this procedure and another group was ventilated with air. After the procedure, the mice were killed and ventilated with pure N(2), after which the trachea was tied off and the lungs were imaged with micro-computed tomography (micro-CT). RESULTS: H was significantly higher in allergic mice than in control animals after methacholine challenge. The ratio of H at the end of the measurement period between allergic and nonallergic mice ventilated with O(2) was 1.36, indicating substantial derecruitment in the allergic animals. The ratio between lung volumes determined by micro-CT in the control and the allergic mice was also 1.36, indicative of a corresponding volume loss due to absorption atelectasis. Micro-CT images and histograms of Hounsfield units from the lungs also showed increased volume loss in the allergic mice compared with control animals after methacholine challenge. CONCLUSIONS: These results support the conclusion that airway closure is a major component of hyperresponsiveness in allergically inflamed mice. |
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Authors:
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Lennart K A Lundblad; John Thompson-Figueroa; Gilman B Allen; Lisa Rinaldi; Ryan J Norton; Charles G Irvin; Jason H T Bates |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-01-25 |
Journal Detail:
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Title: American journal of respiratory and critical care medicine Volume: 175 ISSN: 1073-449X ISO Abbreviation: Am. J. Respir. Crit. Care Med. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-04 Completed Date: 2007-06-05 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9421642 Medline TA: Am J Respir Crit Care Med Country: United States |
Other Details:
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Languages: eng Pagination: 768-74 Citation Subset: AIM; IM |
Affiliation:
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Vermont Lung Center, The University of Vermont College of Medicine, HSRF 230, 149 Beaumont Avenue, Burlington, VT 05405-0075, USA. lennart.lundblad@uvm.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bronchial Hyperreactivity / etiology, physiopathology*, radiography Bronchial Provocation Tests Female Lung Volume Measurements Mice Mice, Inbred BALB C Pulmonary Atelectasis / complications, physiopathology*, radiography Respiration, Artificial Respiratory Hypersensitivity / complications, physiopathology*, radiography Tomography, X-Ray Computed |
| Grant Support | |
ID/Acronym/Agency:
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P20 RR15557/RR/NCRR NIH HHS; R01 HL67273/HL/NHLBI NIH HHS |
| Comments/Corrections | |
Comment In:
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Am J Respir Crit Care Med. 2008 May 15;177(10):1171; author reply 1171-2
[PMID:
18460463
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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