| Airlift-driven fibrous-bed bioreactor for continuous production of glucoamylase using immobilized recombinant yeast cells. | |
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MedLine Citation:
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PMID: 19539672 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Continuous production of a fungal glucoamylase by immobilized recombinant Saccharomyces cerevisiae strain C468 containing plasmid pGAC9. Yeast cells were immobilized on hydrophilic cotton cloth in an inverse internal loop airlift-driven bioreactor. Free-cell culture in the airlift and stirred tank bioreactors confirmed the plasmid instability of the recombinant yeast. Enhanced glucoamylase productivity and plasmid stability were observed both in the free and immobilized cell cultures in the airlift bioreactor system. The glucoamylase level of the free-cell culture in the airlift bioreactor was approximately 20% higher than that in the in stirred tank bioreactor due to high cell density (cell dry weight/volume of bioreactor) and fraction of the plasmid-carrying cells. A potentially high glucoamylase activity of 161U/L and a corresponding volumetric productivity of 3.5U/Lh were achieved when a cell density of approximately 85g/L (or 12.3g/g fiber) was attained in the fibrous-bed immobilized cell bioreactor system. The stable glucoamylase production was achieved after five generations, at which time a fraction of approximately 62% of the plasmid-carrying cells was realized in the immobilized cell system. Plasmid stability was increased for the immobilized cells during continuous culture at the operating dilution rate. The volumetric and specific productivities and fraction of plasmid-carrying cells in the immobilized cell system were higher than in the free-cell counterpart, however. This was in part due to the high viability (approximately 80%) in the immobilized cell system and the selective immobilization of the plasmid-carrying cells in the fibrous bed, and perhaps increased plasmid copy number. |
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Authors:
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Peter Kilonzo; Argyrios Margaritis; Maurice Bergougnou |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-17 |
Journal Detail:
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Title: Journal of biotechnology Volume: 143 ISSN: 1873-4863 ISO Abbreviation: J. Biotechnol. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-28 Completed Date: 2009-11-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8411927 Medline TA: J Biotechnol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 60-8 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biochemical Engineering, University of Western Ontario, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspergillus
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enzymology Biomass Bioreactors* Biotechnology / methods Cell-Free System Equipment Design Ethanol / chemistry Fermentation Glucan 1,4-alpha-Glucosidase / biosynthesis*, chemistry Glucose / chemistry Industrial Microbiology / methods Microscopy, Electron, Scanning / methods Plasmids / metabolism Saccharomyces cerevisiae / enzymology, metabolism* Time Factors |
| Chemical | |
Reg. No./Substance:
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50-99-7/Glucose; 64-17-5/Ethanol; EC 3.2.1.3/Glucan 1,4-alpha-Glucosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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