Document Detail

Airborne endotoxin predicts symptoms in non-mouse-sensitized technicians and research scientists exposed to laboratory mice.
MedLine Citation:
PMID:  12663339     Owner:  NLM     Status:  MEDLINE    
Research scientists, laboratory technicians, and animal handlers who work with animals frequently report respiratory and skin symptoms from exposure to laboratory animals (LA). However, on the basis of prick skin tests or RASTs, only half are sensitized to LA. We hypothesized that aerosolized endotoxin from mouse work is responsible for symptoms in nonsensitized workers. We performed a cross-sectional study of 269/310 (87%) workers at a research institution. Subjects completed a questionnaire and underwent prick skin tests (n = 254) or RASTs (n = 16) for environmental and LA allergens. We measured airborne mouse allergen and endotoxin in the animal facility and in research laboratories. Of 212 workers not sensitized to mice, 34 (16%) reported symptoms compared with 26 (46%) of mouse-sensitized workers (p < 0.001). Symptomatic workers were more likely to be atopic, regardless of mouse sensitization status. Symptomatic non-mouse-sensitized workers spent more time performing animal experiments in the animal facility (p = 0.0001) and in their own laboratories (p < 0.0001) and had higher daily endotoxin exposure (p = 0.008) compared with asymptomatic coworkers. In a multivariate model, daily endotoxin exposure most strongly predicted symptoms to mice in non-mouse-sensitized workers (odds ratio = 30.8, p = 0.003). We conclude that airborne endotoxin is associated with respiratory symptoms to mice in non-mouse-sensitized scientists and technicians.
Karin A Pacheco; Charles McCammon; Andrew H Liu; Peter S Thorne; Marsha E O'Neill; John Martyny; Lee S Newman; Richard F Hamman; Cecile S Rose
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  167     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-03-28     Completed Date:  2003-05-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  983-90     Citation Subset:  AIM; IM    
Department of Medicine, Division of Environmental and Occupational Health Sciences, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA.
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MeSH Terms
Air Pollutants, Occupational / adverse effects*,  immunology
Allergens / administration & dosage,  adverse effects,  immunology
Animal Technicians*
Animals, Laboratory*
Cohort Studies
Cross-Sectional Studies
Dose-Response Relationship, Immunologic
Endotoxins / administration & dosage,  adverse effects*,  immunology
Laboratory Personnel*
Middle Aged
Multivariate Analysis
Predictive Value of Tests
Radioallergosorbent Test
Respiratory Hypersensitivity / diagnosis,  epidemiology,  etiology
Risk Factors
Skin Tests
Statistics as Topic
Grant Support
1F32 AI 10622-01/AI/NIAID NIH HHS; P30 ES 05605/ES/NIEHS NIH HHS
Reg. No./Substance:
0/Air Pollutants, Occupational; 0/Allergens; 0/Endotoxins

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