| Air-exposure technique for the formation of artificial lipid bilayers in microsystems. | |
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MedLine Citation:
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PMID: 17585789 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To develop a reliable method for on-chip bilayer lipid membrane (BLM) formation, which could be employed for use in a biosensor array platform, a polymer microfluidic device has been constructed, and the formation of suspended BLMs within it has been investigated. A simple, yet reproducible BLM formation protocol has been developed, in which a brief air-exposure period is employed to induce the rapid thinning of an initially thick lipid-solvent layer. The technique is rapid, reproducible, and amenable to the simple injection of proteins or analytes, as well as to buffer exchange on both sides of the membrane. Scaling up the technique for use in an array platform is also straightforward, the simultaneous formation of three individually addressable BLMs being demonstrated. |
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Authors:
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Mairi E Sandison; Michele Zagnoni; Hywel Morgan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-06-22 |
Journal Detail:
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Title: Langmuir : the ACS journal of surfaces and colloids Volume: 23 ISSN: 0743-7463 ISO Abbreviation: Langmuir Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-07-10 Completed Date: 2007-10-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882736 Medline TA: Langmuir Country: United States |
Other Details:
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Languages: eng Pagination: 8277-84 Citation Subset: IM |
Affiliation:
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School of Electronics and Computer Science, University of Southampton, Southampton, United Kingdom. mes@ecs.soton.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Air Biosensing Techniques* Lipid Bilayers* Microfluidic Analytical Techniques* Phase Transition* |
| Chemical | |
Reg. No./Substance:
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0/Lipid Bilayers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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