Document Detail


Agrobacterium-mediated transformation of Solanum phureja.
MedLine Citation:
PMID:  15666166     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A population of transgenic plants was produced by the transformation of internodal explants of Solanum phureja, DB337/37 (the cultivar Mayan Gold) using an Agrobacterium tumefaciens LBA4404-based vector containing a phytoene synthase gene (crtB). The regeneration strategy utilised a two-step protocol, with a 12-day callus induction stage mediated by 1.07 microM alpha-napthaleneacetic acid (NAA), 7.10 microM zeatin riboside and 0.06 microM gibberellic acid (GA3), followed by a prolonged (up to 90 day) shoot induction stage on medium containing 0.11 microM NAA, 7.10 microM zeatin riboside and 0.06 microM GA3 supplemented with kanamycin at 50 mg l(-1) as the selection agent. Southern analysis of the transgenic population revealed that the transgene copy number varied between one and five in the lines tested. Northern blot analysis showed significant expression of the introduced crtB gene in some lines during tuber development. Cytological analysis of the material showed a high incidence of chromosome doubling in the transgenic population with over 80% of all lines tested having doubled their chromosome complement during the transformation process.
Authors:
L J M Ducreux; W L Morris; M A Taylor; S Millam
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-01-22
Journal Detail:
Title:  Plant cell reports     Volume:  24     ISSN:  0721-7714     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-30     Completed Date:  2005-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  10-4     Citation Subset:  IM    
Affiliation:
Quality, Health and Nutrition Programme, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK. L.Ducreux@scri.sari.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Carotenoids / biosynthesis
Culture Techniques
Genes, Plant
Genetic Engineering / methods*
Genetic Vectors
Plant Shoots / physiology
Plants, Genetically Modified
Rhizobium radiobacter
Solanum / genetics*
Transformation, Genetic*
Chemical
Reg. No./Substance:
36-88-4/Carotenoids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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