Document Detail


Agrobacteria lacking ornithine lipids induce more rapid tumour formation.
MedLine Citation:
PMID:  22958119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ornithine lipids (OLs) are phosphorus-free membrane lipids that are widespread among Gram-negative bacteria. Their basic structure consists of a 3-hydroxy fatty acyl group attached in amide linkage to the α-amino group of ornithine and a second fatty acyl group ester-linked to the 3-hydroxy position of the first fatty acid. It has been shown that OLs can be hydroxylated within the amide-linked fatty acyl moiety, the secondary fatty acyl moiety or within the ornithine moiety. These modifications have been related to increased stress tolerance and symbiotic proficiency in different organisms such as Rhizobium tropici or Burkholderia cenocepacia. Analysing the membrane lipid composition of the plant pathogen Agrobacterium tumefaciens we noticed that it forms two different OLs. In the present work we studied if OLs play a role in stress tolerance and pathogenicity in A. tumefaciens. Mutants deficient in the OLs biosynthesis genes olsB or olsE were constructed and characterized. They either completely lack OLs (ΔolsB) or only form the unmodified OL (ΔolsE). Here we present a characterization of both OL mutants under stress conditions and in a plant transformation assay using potato tuber discs. Surprisingly, the lack of agrobacterial OLs promotes earlier tumour formation on the plant host.
Authors:
Miguel Ángel Vences-Guzmán; Ziqiang Guan; José Roberto Bermúdez-Barrientos; Otto Geiger; Christian Sohlenkamp
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-09-09
Journal Detail:
Title:  Environmental microbiology     Volume:  15     ISSN:  1462-2920     ISO Abbreviation:  Environ. Microbiol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-05     Completed Date:  2013-11-26     Revised Date:  2013-12-05    
Medline Journal Info:
Nlm Unique ID:  100883692     Medline TA:  Environ Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  895-906     Citation Subset:  IM    
Copyright Information:
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.
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MeSH Terms
Descriptor/Qualifier:
Agrobacterium / genetics*,  metabolism*,  pathogenicity
Lipids / genetics
Membrane Lipids / chemistry,  metabolism
Ornithine / analogs & derivatives*,  genetics,  metabolism
Plant Tubers / microbiology
Plant Tumors / microbiology*
Solanum tuberosum / microbiology
Stress, Physiological
Grant Support
ID/Acronym/Agency:
GM-069338/GM/NIGMS NIH HHS; U54 GM069338/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Lipids; 0/Membrane Lipids; 0/ornithine containing aminolipid; E524N2IXA3/Ornithine
Comments/Corrections

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