Document Detail

Agreement between theory and measurement in quantification of ammonia-oxidizing bacteria.
MedLine Citation:
PMID:  16204554     Owner:  NLM     Status:  MEDLINE    
Autotrophic ammonia-oxidizing bacteria (AOB) are of vital importance to wastewater treatment plants (WWTP), as well as being an intriguing group of microorganisms in their own right. To date, corroboration of quantitative measurements of AOB by fluorescence in situ hybridization (FISH) has relied on assessment of the ammonia oxidation rate per cell, relative to published values for cultured AOB. Validation of cell counts on the basis of substrate transformation rates is problematic, however, because published cell-specific ammonia oxidation rates vary by over two orders of magnitude. We present a method that uses FISH in conjunction with confocal scanning laser microscopy to quantify AOB in WWTP, where AOB are typically observed as microcolonies. The method is comparatively simple, requiring neither detailed cell counts or image analysis, and yet it can give estimates of either cell numbers or biomass. Microcolony volume and diameter were found to have a log-normal distribution. We were able to show that virtually all (>96%) of the AOB biomass occurred as microcolonies. Counts of microcolony abundance and measurement of their diameter coupled with a calibration of microcolony dimensions against cell numbers or AOB biomass were used to determine AOB cell numbers and biomass in WWTP. Cell-specific ammonia oxidation rates varied between plants by over three orders of magnitude, suggesting that cell-specific ammonia oxidation is an important process variable. Moreover, when measured AOB biomass was compared with process-based estimates of AOB biomass, the two values were in agreement.
Gulnur Coskuner; Stuart J Ballinger; Russell J Davenport; Rheanne L Pickering; Rosario Solera; Ian M Head; Thomas P Curtis
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  71     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-05     Completed Date:  2005-12-02     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6325-34     Citation Subset:  IM    
School of Civil Engineering and Geosciences, University of Newcastle upon Tyne, Newcastle NE1 7RU, United Kingdom.
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MeSH Terms
Ammonia / metabolism*
Bacteria / genetics,  isolation & purification*
Colony Count, Microbial
In Situ Hybridization, Fluorescence / methods*
Microscopy, Confocal / methods*
Oligonucleotide Probes
Sewage / microbiology*
Waste Disposal, Fluid / methods
Reg. No./Substance:
0/Oligonucleotide Probes; 0/Sewage; 7664-41-7/Ammonia

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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