Document Detail


Agonistic and antagonistic effects of zearalenone, an etrogenic mycotoxin, on SKN, HHUA, and HepG2 human cancer cell lines.
MedLine Citation:
PMID:  11205083     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Zearalenone (ZEA) is a nonsteroidal estrogenic compound mainly produced by the molds Fusarium graminearium and Fusarium culmorum found in a variety of host plants and soil debris around the world. ZEA is usualy non-lethal to animals but is important to livestock producers because its hyperestrogenic effects adversely influence the reproductive performance of animals. There have been suggestions of possible involvement of ZEA in the progression of breast malignancies and tumors of the female reproductive tract in humans. The toxic or stimulatory effects of ZEA and its metabolites alpha-zearalenol and 17-beta-estradiol on SKN, HHUAand HepG2 cells were studied using rapid colorimetric MTT assay. In general, both concentrations of 17-beta-estradiol (100M and 10 nM) were toxic to SKN and HHUA cell cultures. Both ZEA and alpha-zearalenol stimulated the proliferation of SKN and HHUA cells. On HepG2 cells, lower concentrations (10 nM) of 17-beta-estradiol and higher concentrations (100 microM) of ZEA exhibited toxic effects, whereas treatment with higher concentrations of 17-beta-estradiol and lower concentration of ZEA did not show toxic effects. A dose dependent antagonistic effect was observed when the cell cultures were pre-incubated with ICI 182,780, a synthetic estrogen receptor blocker, before estradiol or mycotoxin treatments.
Authors:
G S Withanage; H Murata; T Koyama; I Ishiwata
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Veterinary and human toxicology     Volume:  43     ISSN:  0145-6296     ISO Abbreviation:  Vet Hum Toxicol     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-01     Completed Date:  2001-03-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7704194     Medline TA:  Vet Hum Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6-10     Citation Subset:  IM    
Affiliation:
Feed Safety Research Division, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / drug therapy*
Carcinoma, Hepatocellular / drug therapy*
Cell Division / drug effects
Colorimetry
Dose-Response Relationship, Drug
Endometrial Neoplasms / drug therapy*
Estradiol / pharmacology*
Estrogens, Non-Steroidal / agonists*,  metabolism,  therapeutic use
Female
Humans
Leiomyosarcoma / drug therapy*
Tumor Cells, Cultured / drug effects*
Zearalenone / agonists*,  metabolism,  therapeutic use
Zeranol / analogs & derivatives,  therapeutic use*
Chemical
Reg. No./Substance:
0/Estrogens, Non-Steroidal; 0/zearalenol; 17924-92-4/Zearalenone; 26538-44-3/Zeranol; 50-28-2/Estradiol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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