| Agonist-induced internalization of κ-opioid receptors in noradrenergic neurons of the rat locus coeruleus. | |
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MedLine Citation:
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PMID: 20884346 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Kappa-opioid receptors (κOR) are positioned to modulate pre- and post-synaptic responses of norepinephrine-containing neurons in the rat locus coeruleus (LC). The ability of an acute systemic injection of a long acting κOR agonist, U50,488, to induce trafficking of κOR was assessed in the LC using immunogold-silver detection in male Sprague-Dawley rats. U50,488 administration shifted immunogold-silver labeling indicative of κOR from primarily plasmalemmal sites to intracellular sites when compared to vehicle-treated subjects. This translocation from the plasma membrane to the cytoplasmic compartment was prevented by pre-treatment with the κOR antagonist, norbinaltorphimine (norBNI). To determine whether agonist stimulation could induce adaptations in the expression of the noradrenergic synthesizing enzyme, dopamine beta hydroxylase (DβH), and κOR expression, Western blot analysis was used to compare expression levels of DβH and κOR following U50,488 administration. Expression levels for DβH and κOR were significantly increased following U50,488 administration when compared to controls. These data indicate that a systemic injection of a κOR agonist stimulates internalization of κORs in noradrenergic neurons and can impact κOR and DβH expression levels in this stress-sensitive brain region. |
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Authors:
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B A S Reyes; Charles Chavkin; E J Van Bockstaele |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-09-25 |
Journal Detail:
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Title: Journal of chemical neuroanatomy Volume: 40 ISSN: 1873-6300 ISO Abbreviation: J. Chem. Neuroanat. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-24 Completed Date: 2011-11-07 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 8902615 Medline TA: J Chem Neuroanat Country: Netherlands |
Other Details:
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Languages: eng Pagination: 301-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Neuroscience, Farber Institute for Neurosciences, Thomas Jefferson University, 900 Walnut Street, Philadelphia, PA 19107, USA. Beverly.Reyes@jefferson.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer
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administration & dosage Animals Dopamine beta-Hydroxylase / biosynthesis, genetics Endocytosis / drug effects, physiology Enkephalins / biosynthesis, genetics Locus Coeruleus / drug effects*, metabolism*, ultrastructure Male Microscopy, Immunoelectron Neurons / drug effects*, metabolism*, ultrastructure Norepinephrine / physiology Protein Precursors / biosynthesis, genetics Rats Rats, Sprague-Dawley Receptors, Opioid, kappa / agonists*, metabolism*, ultrastructure |
| Grant Support | |
ID/Acronym/Agency:
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DA 09082/DA/NIDA NIH HHS; K05 DA020570/DA/NIDA NIH HHS; R01 DA009082-14/DA/NIDA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Enkephalins; 0/Protein Precursors; 0/Receptors, Opioid, kappa; 51-41-2/Norepinephrine; 67198-13-4/3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer; 93443-35-7/preproenkephalin; EC 1.14.17.1/Dopamine beta-Hydroxylase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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