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Agmatine enhances neurogenesis by increasing ERK1/2 expression, and suppresses astrogenesis by decreasing BMP 2,4 and SMAD 1,5,8 expression in subventricular zone neural stem cells.
MedLine Citation:
PMID:  21843531     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIM: Our study aimed to demonstrate whether agmatine could regulate proliferation and cell fate determination of subventricular zone neural stem cells (SVZ NSCs). MAIN METHODS: SVZ NSCs were grown in the presence of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) (20ng/ml) until 4days in vitro (DIV) and later the culture medium was replaced without EGF and bFGF until 11 DIV in the absence (EGF/bFGF(+/-)/Ag(-)) or presence of agmatine (EGF/bFGF(+/-)/Ag(+)). Another set SVZ NSCs were maintained with EGF and bFGF until 11 DIV without (EGF/bFGF(+/+)/Ag(-)) or with agmatine treatment (EGF/bFGF(+/+)/Ag(+)). Agmatine's effect on proliferation and cell death (H and PI staining and Caspase-3 immunostaining) was examined at DIV 4 and 11. Agmatine's (100μM) effect on cell fate determination was confirmed by immunostaining and Western blot at 11 DIV. KEY FINDINGS: Agmatine treatment reduced the neurosphere size and total cell count number dose-dependently in all the experimental groups both at DIV 4 and11. Immunoblotting and staining results showed that agmatine increased the Tuj1 and MAP2 and decreased the GFAP with no change in the Oligo2 protein expressions. This neurogenesis effect of agmatine seems to have a relation with ERK1/2 activation and anti-astrogenesis effect is thought to be related with the suppression of BMP 2,4 and SMAD 1,5,8 protein expression. SIGNIFICANCE: This model could be an invaluable tool to study whether agmatine treated SVZ NSC transplantation to the CNS injury could trigger neurogenesis and decrypt the full range of molecular events involved during neurogenesis in vivo as evidenced in vitro.
Authors:
Ho Won Song; Bokara Kiran Kumar; Sa Hyun Kim; Yong Heui Jeon; Yoon A Lee; Won Teak Lee; Kyung Ah Park; Jong Eun Lee
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-27
Journal Detail:
Title:  Life sciences     Volume:  -     ISSN:  1879-0631     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-8-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Department of Anatomy, Yonsei University College of Medicine, 250 Seongsanno, Seodaemun-gu, Seoul 120-752, South Korea.
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