| Agkistin-s, a disintegrin domain, inhibits angiogenesis and induces BAECs apoptosis. | |
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MedLine Citation:
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PMID: 16924676 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous work in our laboratory has shown agkistin, a snake venom metalloproteases (SVMPs) from the venom of Agkistrodon halys, possesses antiplatelet aggregation activity. In this study, we further examined the antiangiogenic activity of agkistin-s, the disintegrin domain of agkistin. Recombinant agkistin-s was produced in Escherichia coli by subcloning its cDNA into pET28a vector, and the effect of purified agkistin-s was evaluated. At the concentration of 0.5-1.5 microM, the recombinant agkistin-s exhibited inhibitory activities on the bovine aortic endothelial cells (BAECs) migration and proliferation in a dose-dependent manner. In addition, it exhibited an effective antiangiogenic effect when assayed by using the 10-day-old embryo chick CAM model and effectively inhibits the tube-like structure formation. Furthermore, it potently induced BAECs apoptosis as examined by flow cytometric assays. |
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Authors:
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Aixia Ren; Shaohui Wang; Wenjie Cai; Guanzhen Yang; Yichun Zhu; Xiangfu Wu; Yaozhou Zhang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 99 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-11-27 Completed Date: 2007-01-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 1517-23 Citation Subset: IM |
Copyright Information:
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(c) 2006 Wiley-Liss, Inc. |
Affiliation:
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Institute of Biochemistry and Biotechnology, College of Life Sciences, Zhejiang University, Hangzhou 310029, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Cattle Cells, Cultured Chickens Endothelium, Vascular / cytology, drug effects* Neovascularization, Pathologic* Recombinant Proteins / pharmacology Viper Venoms / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins; 0/Viper Venoms; 0/agkistin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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