Document Detail


Agitation and aeration effects in suspension mammalian cell cultures.
MedLine Citation:
PMID:  22358730     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m(-2). Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effects at the reactor level have been included here. A discussion of the difference between suspension and microcarrier cultures, in relation to hydrodynamic effects, is included.
Authors:
C P Marquis; K S Low; J P Barford; C Harbour
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytotechnology     Volume:  2     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  1989 Aug 
Date Detail:
Created Date:  2012-02-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  163-70     Citation Subset:  -    
Affiliation:
Dept. of Infectious Diseases, The University of Sydney, 2006, NSW, Australia.
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