Document Detail

Aging sensitizes rapidly isolated hippocampal microglia to LPS ex vivo.
MedLine Citation:
PMID:  20537730     Owner:  NLM     Status:  MEDLINE    
The present study tested whether aging sensitizes hippocampal microglia to a pro-inflammatory challenge ex vivo. Hippocampal microglia from 3 and 24 mo old male F344 x BN F1 rats were exposed to LPS (0, 0.1, 1, 10 and 100 ng/ml) ex vivo. 2 h post-LPS challenge, gene expression of microglial activation markers and cytokines were assessed. 24 mo old animals exhibited a potentiated pro-inflammatory cytokine (IL-1β and IL-6) response to LPS and increased levels of CD11b, Iba-1 and MHCII irrespective of LPS treatment. The present results demonstrate that aging sensitizes hippocampal microglia to pro-inflammatory challenges.
Matthew G Frank; Ruth M Barrientos; Linda R Watkins; Steven F Maier
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-05-26
Journal Detail:
Title:  Journal of neuroimmunology     Volume:  226     ISSN:  1872-8421     ISO Abbreviation:  J. Neuroimmunol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-10     Completed Date:  2010-10-06     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  8109498     Medline TA:  J Neuroimmunol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  181-4     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
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MeSH Terms
Aging / immunology*
Antigens, CD11b / genetics,  metabolism
Calcium-Binding Proteins / genetics,  metabolism
Cytokines / genetics,  metabolism
Dose-Response Relationship, Drug
Gene Expression Regulation / drug effects
Hippocampus / cytology*,  drug effects
Histocompatibility Antigens Class II / genetics,  metabolism
Lipopolysaccharides / pharmacology*
Microfilament Proteins
Neuroglia / drug effects*,  immunology
Rats, Inbred F344
Grant Support
AG028271/AG/NIA NIH HHS; R01 AG028271/AG/NIA NIH HHS; R01 AG028271-05/AG/NIA NIH HHS
Reg. No./Substance:
0/Aif1 protein, rat; 0/Antigens, CD11b; 0/Calcium-Binding Proteins; 0/Cytokines; 0/Histocompatibility Antigens Class II; 0/Lipopolysaccharides; 0/Microfilament Proteins

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