Document Detail


Aging differentially affects human skeletal muscle amino acid transporter expression when essential amino acids are ingested after exercise.
MedLine Citation:
PMID:  22889597     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND & AIMS: Amino acid transporters have been proposed as regulators of protein synthesis. The primary aim of this study was to determine whether amino acid transporter expression is increased in human muscle following resistance exercise (RE) coupled with essential amino acid (EAA) ingestion, and whether a differential response occurs with aging. Secondly, we aimed to compare this response to a previous study examining RE alone.
METHODS: Young (n = 7, 30 ± 2 yr) and older men (n = 6, 70 ± 2 yr) ingested EAA 1 h after RE. Muscle biopsies were obtained at rest and 3 and 6 h post exercise to examine amino acid transporter mRNA and protein expression.
RESULTS: In both age groups, RE + EAA increased mRNA of L-type amino acid transporter 1 (LAT1)/solute linked carrier (SLC)7A5, sodium-coupled neutral amino acid transporter 2 (SNAT2)/SLC38A2, and cationic amino acid transporter 1/SLC7A1 (p < 0.05). SNAT2 protein increased in young at 3 and 6 h (p < 0.05), whereas old maintained higher LAT1 protein (p < 0.05). Compared to RE alone, RE + EAA enhanced amino acid transporter expression only in young (p < 0.05).
CONCLUSIONS: RE increases muscle amino acid transporter expression in young and older adults, however, post exercise EAA ingestion enhances amino acid transporter expression only in young indicating that aging may influence the function of specific amino acid transporters.
Authors:
Jared M Dickinson; Micah J Drummond; Jennifer R Coben; Elena Volpi; Blake B Rasmussen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-08-01
Journal Detail:
Title:  Clinical nutrition (Edinburgh, Scotland)     Volume:  32     ISSN:  1532-1983     ISO Abbreviation:  Clin Nutr     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-11     Completed Date:  2013-09-23     Revised Date:  2014-05-28    
Medline Journal Info:
Nlm Unique ID:  8309603     Medline TA:  Clin Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  273-80     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aging*
Amino Acid Transport Systems / genetics,  metabolism*
Amino Acids, Essential / administration & dosage*,  blood
Blood Glucose / analysis
Exercise / physiology*
Humans
Leucine / blood
Male
Muscle Proteins / biosynthesis
Muscle, Skeletal / metabolism*
Protein Biosynthesis
RNA, Messenger / genetics,  metabolism
Rest / physiology
Grant Support
ID/Acronym/Agency:
P30 AG024832/AG/NIA NIH HHS; P30AG024832/AG/NIA NIH HHS; R01 AG018311/AG/NIA NIH HHS; R01 AR049877/AR/NIAMS NIH HHS; R01AG018311/AG/NIA NIH HHS; R01AR049877/AR/NIAMS NIH HHS; T35 AG038048/AG/NIA NIH HHS; T35AG038048/AG/NIA NIH HHS; UL1 TR000071/TR/NCATS NIH HHS; UL1TR000071/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acid Transport Systems; 0/Amino Acids, Essential; 0/Blood Glucose; 0/Muscle Proteins; 0/RNA, Messenger; GMW67QNF9C/Leucine
Comments/Corrections

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