Document Detail


Aging changes of pancreatic isoamylases and the appearance of "old amylase" in the serum of patients with pancreatic pseudocysts.
MedLine Citation:
PMID:  6160076     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human serum and pancreatic secretions contain at least three isoamylases of pancreatic origin. As determined by gel electrophoresis and saccharogenic amylase assay, P1, P2, and P3 designate isoenzymes with slow, intermediate, and rapid electrophoretic mobilities, respectively. The P1 isoamylase normally accounts fo 80-90% of total amylase activity, P2 for 10-20%, and P3 for 0-4% in both serum and pancreatic juice. When pancreatic amylase is incubated at 37 degrees C, P1 decreases, and P2 and P3 increase within hours. Whereas P2/P1 is always < 0.25 in fresh pancreatic juice, normal serum, acute pancreatitis serum, chronic pancreatitis serum, or pancreatic cancer serum, the ratio was elevated in 11 of 12 pseudocyst contents (mean P2/P1 = 0.51) and in 14 of 16 sera from patients with proven pseudocysts (mean P2/P1 = 0.43) (P < 0.001). After pseudocysts were surgically drained, the proportions of the pancreatic isoamylases in serum reverted to normal. The precise, characteristic, and predictable changes in electrophoretic mobility, presumably a result of specific spontaneous chemical alterations of the isoenzyme molecules, allow identification of "old amylase" in the serum of patients with pancreatic pseudocysts. This finding may be a useful adjunct for diagnosis, but whether the amount of "old amylase" can be used to estimate the age of a pseudocyst is not yet known.
Authors:
A L Warshaw; K H Lee
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Gastroenterology     Volume:  79     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  1980 Dec 
Date Detail:
Created Date:  1981-02-19     Completed Date:  1981-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1246-51     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Amylases / blood*
Chronic Disease
Diagnosis, Differential
Electrophoresis, Polyacrylamide Gel
Glycoside Hydrolases / blood*
Humans
Isoamylase / blood*,  isolation & purification
Pancreas / enzymology*
Pancreatic Cyst / enzymology*
Pancreatic Neoplasms / enzymology
Pancreatic Pseudocyst / diagnosis,  enzymology*
Pancreatitis / enzymology
Time Factors
Chemical
Reg. No./Substance:
EC 3.2.1.-/Amylases; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.68/Isoamylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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