Document Detail


Aggregation substance of Enterococcus faecalis mediates adhesion to cultured renal tubular cells.
MedLine Citation:
PMID:  1729187     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sex pheromone system of Enterococcus faecalis is a unique, highly efficient plasmid collection mechanism for this species. A crucial role in this system is played by an adhesin called aggregation substance which enables the cell-cell contact between donor and recipient strains. The existence of the amino acid motif Arg-Gly-Asp-Ser in the adhesin prompted us to look for a possible binding of E. faecalis cells expressing aggregation substance to eucaryotic cells. We were able to show that the adhesin mediated binding to cultured renal tubular cells (porcine cell line LLC-PK1) via light microscopic, electron microscopic, and enzyme-linked immunosorbent assay-based studies. Synthesis of the adhesin was induced by some component(s) of serum. These data are interpreted to mean that aggregation substance is an adhesin mediating not only cell-cell contact between different E. faecalis strains but also binding of E. faecalis to eucaryotic cells, and therefore it might contribute to virulence.
Authors:
B Kreft; R Marre; U Schramm; R Wirth
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  60     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1992 Jan 
Date Detail:
Created Date:  1992-02-12     Completed Date:  1992-02-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  25-30     Citation Subset:  IM    
Affiliation:
Klinik für Innere Medizin, Medizinische Universität zu Lübeck, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Bacterial Adhesion / physiology*
Blotting, Western
Cell Adhesion Molecules*
Cell Communication / physiology
Cells, Cultured
Enterococcus faecalis / pathogenicity*
Enzyme-Linked Immunosorbent Assay
Gene Expression Regulation, Bacterial
Kidney Tubules / microbiology*
Microscopy, Electron
Molecular Sequence Data
Oligopeptides / physiology
Proteins / physiology*
Swine
Chemical
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/Oligopeptides; 0/Proteins; 0/cell aggregation factors; 94495-17-7/cAD1 bacterial sex hormone
Comments/Corrections

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