Document Detail

Aggregation and sedimentation of mixtures of erythrocytes with different properties.
MedLine Citation:
PMID:  11847413     Owner:  NLM     Status:  MEDLINE    
The rouleau formation of erythrocytes and the erythrocyte sedimentation were examined for mixture of different kinds of the cells suspended in isotonic phosphate-buffered saline containing 1 or 2 g/dl dextran T-70 (MW = 70,400) and 4 g/dl albumin, using a low shear rheoscope and the Westergren method, respectively. The deformability of cells was decreased by treating with diamide, diazene-dicarboxylic acid bis[N,N-dimethylamide], and the sialic acid content of cells, i.e., the surface negative charge, was reduced by treating with neuraminidase. (1) The rate of rouleau formation was decreased in cells with decreased deformability, while it was increased in cells with reduced sialic acid content. The rate changed in proportion to the ratio of the modified cells to normal cells. (2) The erythrocyte sedimentation was also decreased in cells with decreased deformability, while it was increased in cells with reduced sialic acid content. Furthermore, the erythrocyte sedimentation changed almost proportionally to the ratio of the modified cells to normal cells. (3) When normal deformable cells were mixed with cells with decreased deformability, the deformable cells seemed to settle faster than the less deformable cells, though the difference was not significant. (4) When normal cells were mixed with cells with reduced sialic acid content, the cells with less sialic acid settled significantly faster than those with more sialic acid. The present experiment may conclude that erythrocyte aggregation is induced preferentially among more deformable cells and/or among less negatively charged cells with weaker electrostatic repulsive force, and then the formed aggregates settle faster.
Y Suzuki; N Tateishi; I Cicha; N Maeda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical hemorheology and microcirculation     Volume:  25     ISSN:  1386-0291     ISO Abbreviation:  Clin. Hemorheol. Microcirc.     Publication Date:  2001  
Date Detail:
Created Date:  2002-03-06     Completed Date:  2002-05-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9709206     Medline TA:  Clin Hemorheol Microcirc     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  105-17     Citation Subset:  IM    
Department of Physiology, School of Medicine, Ehime University, Shigenobu, Onsen-gun, Ehime 791-0295, Japan.
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MeSH Terms
Blood Sedimentation*
Diamide / pharmacology
Erythrocyte Aggregation*
Erythrocyte Deformability / drug effects
Erythrocyte Indices
Erythrocyte Membrane / chemistry,  drug effects
Erythrocytes / chemistry,  drug effects
N-Acetylneuraminic Acid / blood
Neuraminidase / pharmacology
Spectrin / chemistry
Static Electricity
Reg. No./Substance:
0/Solutions; 10465-78-8/Diamide; 12634-43-4/Spectrin; 131-48-6/N-Acetylneuraminic Acid; EC

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