| Aggregation and fusion of vesicles composed of N-palmitoyl derivatives of membrane phospholipids. | |
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MedLine Citation:
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PMID: 10907786 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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N-Acylphosphatidylethanolamines and N-acylphosphatidylserines have been isolated from mammalian cells and have been associated with some tissue degenerative changes, although the relationship between their synthesis and the uncontrolled sequence of events that ends in irreversible tissue damage is not completely established. Our results show that monovalent and divalent cations induce aggregation and fusion of liposomes constituted by N-palmitoylphosphatidylethanolamine (NPPE) and N-palmitoylphosphatidylserine (NPPS). The effectiveness of cations to induce the aggregation of NPPE and NPPS liposomes is Ca2+ > Mg2+ >> Na+. NPPS liposomes aggregate at lower concentrations of divalent cations than NPPE liposomes, but with sodium NPPE liposomes aggregate to a higher extent than NPPS liposomes. The reaction order for the aggregation processes depends on the lipid and the cation nature and range from 1.04 to 1.64. Dynamic light scattering shows an irreversible increase of the size of the aggregates in the presence of all cations tested. The irreversibility of the aggregation process and the intermixing of bilayer lipids, as studied by resonance energy transfer assay, suggest that fusion, rather than aggregation, occurs. The existence of a real fusion was demonstrated by the coalescence of the aqueous contents of both NPPS and NPPE liposomes in the presence of either monovalent or divalent cations. The different binding sensitivity of Ca2+ to NPPS and NPPE liposomes, determined by zeta potential measurements, agrees with the results obtained in the aggregation and fusion assays. Our results suggest that the synthesis in vivo of N-acylated phospholipids can introduce important changes in membrane-mediated processes. |
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Authors:
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M Mora; F Mir; M A de Madariaga; M L Sagristá |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Lipids Volume: 35 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 2000 May |
Date Detail:
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Created Date: 2000-10-30 Completed Date: 2001-01-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 513-24 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Faculty of Chemistry, University of Barcelona, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calcium
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metabolism Cell Membrane / chemistry, metabolism* Dose-Response Relationship, Drug Drug Delivery Systems Kinetics Light Lipids / chemistry Liposomes / chemistry Magnesium / metabolism Palmitic Acid / metabolism* Phosphatidylserines / metabolism Phospholipids / chemistry*, metabolism* Scattering, Radiation Sodium / metabolism Sodium Chloride / pharmacology Succinimides / metabolism Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Lipids; 0/Liposomes; 0/Phosphatidylserines; 0/Phospholipids; 0/Succinimides; 125709-33-3/N-(3-pyridyl)-3-phenylsuccinimide; 3036-82-6/dipalmitoylphosphatidylserine; 57-10-3/Palmitic Acid; 7439-95-4/Magnesium; 7440-23-5/Sodium; 7440-70-2/Calcium; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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