Document Detail


Aggregation formation mediated anoikis resistance of BEL7402 hepatoma cells.
MedLine Citation:
PMID:  19056537     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Anoikis resistance is the prerequisite of cancer cells metastasis. Elucidation of the mechanism of anoikis resistance remains a significant challenge. We reported here a model to mimic anoikis resistant process of hepatoma cells in vitro. Experimental results indicated cell to cell aggregation could mediate anoikis resistance of BEL7402 hepatoma cells. Further investigation of these aggregations indicated the biological properties changed greatly after the hepatoma cells lost their anchorage. Aggregation forming process could be separated into three distinct phases according to their biological characteristics, comprising of premature phase, mature phase and postmature phase. Mature phase aggregations have the premium state of cell viability and may mimic the metastatic cells in the circulating system. Biological properties of these three phases aggregations were studied in details including morphological alteration, cell viability and microarray expression profiles. It indicated there was a great upregulation of adhesion molecules during the process of aggregation formation and the cell to cell contact in the aggregation may be mediated independent of calcium involved adhesion pathway. This model might shed light on the anoikis resistance mechanism of hepatoma cells and help to develop new therapies that may target the anoikis resistant hepatoma cells in the metastasis process.
Authors:
Zhiyong Zhang; Lihui Han; Lili Cao; Xiaohong Liang; Yugang Liu; Hua Liu; Juan Du; Zhonghua Qu; Changjun Zhu; Suxia Liu; Hui Li; Wensheng Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society     Volume:  46     ISSN:  1897-5631     ISO Abbreviation:  Folia Histochem. Cytobiol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-12-05     Completed Date:  2009-01-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8502651     Medline TA:  Folia Histochem Cytobiol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  331-6     Citation Subset:  IM    
Affiliation:
Institute of Immunology, Shandong University School of Medicine, Jinan 250012, China.
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MeSH Terms
Descriptor/Qualifier:
Anoikis / physiology*
Calcium / metabolism
Carcinoma, Hepatocellular / metabolism,  pathology*
Cell Aggregation
Cell Line, Tumor
Humans
Liver Neoplasms / metabolism,  pathology*
Neoplasm Metastasis
Chemical
Reg. No./Substance:
7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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