| Aggregation of banana pyrophosphate fructose 6-phosphate 1-phosphotransferase by glycerol. | |
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MedLine Citation:
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PMID: 9219436 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Addition of glycerol during purification of banana (Musaceae, Musa cavendishii) pyrophosphate fructose 6-phosphate 1-phosphotransferase [(PFP), EC 2.7.1.90] initiated molecular aggregation of the enzyme. The aggregation process was dependent on the glycerol concentration. The native enzyme (66 kDa molecular mass) showed enhanced activity at 3% (V/V) or less of glycerol concentration. Glycerol concentration between 4 and 5% (V/V) affected a gradual and sequential aggregation of native form of the enzyme. These aggregated forms had molecular masses of 135, 200 and 270 kDa. The 135 and 200 kDa forms were stable for about 72 hrs and prolonged storage over 2 weeks resulted in the formation of the 270 kDa form. Concentration over 5% could reduce the time required for aggregation. Fru2.6 bis P activated the enzyme over ten fold, but did not help in the aggregation process. Studies on the role of glycerol on PFP specific activity suggested a difference in the activation process compared to that by Fru2.6bis P. Replacement of Hepes buffer by Tris increased the Fru2.6 bis P requirement for maximum activation by around 10 fold. Removal of glycerol from the buffer media resulted in almost complete inactivation of the enzyme. |
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Authors:
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K K Surendranathan; S U Kanulkar; P M Nair |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Indian journal of biochemistry & biophysics Volume: 33 ISSN: 0301-1208 ISO Abbreviation: Indian J. Biochem. Biophys. Publication Date: 1996 Dec |
Date Detail:
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Created Date: 1997-08-05 Completed Date: 1997-08-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0310774 Medline TA: Indian J Biochem Biophys Country: INDIA |
Other Details:
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Languages: eng Pagination: 498-505 Citation Subset: IM |
Affiliation:
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Biotechnology Division (FIPLY), Bhabha Atomic Research Centre, Mumbai, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cellulose
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analogs & derivatives Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Enzyme Activation Fructosediphosphates / pharmacology Fruit / enzymology* Glycerol / pharmacology* Molecular Weight Phosphotransferases / chemistry, isolation & purification, metabolism* Protein Conformation |
| Chemical | |
Reg. No./Substance:
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0/Fructosediphosphates; 56-81-5/Glycerol; 79082-92-1/fructose 2,6-diphosphate; 9004-34-6/Cellulose; 9015-14-9/phosphocellulose; EC 2.7.-/Phosphotransferases; EC 2.7.1.90/pyrophosphate-fructose 6-phosphate 1-phosphotransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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