| Aggregating and prostanoid-releasing effects of platelet-activating factor and leukotrienes on human polymorphonuclear leukocytes and platelets. | |
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MedLine Citation:
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PMID: 2981776 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aggregating and prostanoid-releasing properties of the inflammatory mediators, platelet-activating factor (PAF) and leukotrienes B4, C4 and D4 were studied in human polymorphonuclear leukocytes (PMNL) and in human platelet-rich plasma. Leukotriene B4 (LTB4) and PAF both induced a reversible aggregation of human PMNL with concomitant stimulation of PGE2 formation, whereas LTC4 had no effect on human PMNL. Arachidonic acid (AA) caused an irreversible aggregation of PMNL which was accompanied by formation of both PGE2 and TXB2. Inhibition of TXB2 synthesis by indomethacin or by OKY-1581, a thromboxane synthetase inhibitor, had no effect on the PMNL aggregation induced by LTB4, PAF or AA. Leukotrienes B4, C4 and D4 caused neither aggregation nor TXB2 release in human platelet-rich plasma. PAF, on the other hand, induced a dose-dependent, reversible platelet aggregation which was not accompanied by TXB2 formation nor inhibited by OKY-1581. The present study indicates that in addition to inducing PMNL aggregation, LTB4 is capable of releasing arachidonate metabolites from human PMNL but not from human platelets. Also the responses of PMNL and platelets to PAF seem to differ as the PAF-induced PMNL aggregation was accompanied by increased prostanoid formation whereas the PAF-induced reversible platelet aggregation was obviously independent from arachidonate metabolism. |
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Authors:
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M L Dahl |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International archives of allergy and applied immunology Volume: 76 ISSN: 0020-5915 ISO Abbreviation: Int. Arch. Allergy Appl. Immunol. Publication Date: 1985 |
Date Detail:
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Created Date: 1985-03-08 Completed Date: 1985-03-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0404561 Medline TA: Int Arch Allergy Appl Immunol Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 145-50 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Aggregation
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drug effects Dinoprostone Humans Leukotriene B4 / pharmacology Neutrophils / drug effects* Platelet Activating Factor / pharmacology* Platelet Aggregation / drug effects* Prostaglandin-Endoperoxide Synthases / metabolism Prostaglandins E / biosynthesis SRS-A / pharmacology Thromboxane B2 / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Platelet Activating Factor; 0/Prostaglandins E; 0/SRS-A; 363-24-6/Dinoprostone; 54397-85-2/Thromboxane B2; 71160-24-2/Leukotriene B4; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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