Document Detail


Agglutinin-mediated phagocytosis-associated generation of superoxide anion and nitric oxide by the hemocytes of the giant freshwater prawn Macrobrachium rosenbergii.
MedLine Citation:
PMID:  18222707     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hemocyte mediated phagocytosis is one of the vital components of innate defence mechanisms in crustaceans and this phagocytic process is aided by serum agglutinins. However, literature on agglutinin mediated opsono-phagocytosis is unclear in the case of Macrobrachium rosenbergii hemocytes. Further, very few studies in the case of superoxide anion generation and none with regard to nitric oxide generation during phagocytosis exist among crustaceans. We investigated the occurrence of agglutinins in the serum and the role of serum agglutinins in mediating phagocytosis by the hemocytes. We show that the prawn serum possesses agglutinins that function as opsonins during phagocytosis of HB RBC by the hemocytes. Hemagglutination-inhibition assays revealed the specificity of serum agglutinins for N-acetylated hexoses, namely GalNAc, GlcNAc and ManNAc, with a higher affinity for ManNAc. In addition, ManNAc was able to inhibit the phagocytic response (by about 60%) of the hemocytes against serum pretreated HB RBC, wherein the serum was previously treated with ManNAc. We next investigated the ability of the hemocytes to generate superoxide anion and nitric oxide during HB RBC phagocytosis and results show generation of both these free radicals. In addition, there was an enhancement in generation (75% increase) of these free radicals during agglutinin mediated opsonophagocytosis, when compared to buffer treated targets and interestingly this enhanced generation was inhibited by ManNAc (27% for superoxide anion and 36% for nitric oxide), an inhibitory sugar for phagocytosis. Inhibition of phagocytosis induced superoxide anion generation by DPI (53%), sodium azide (56%) and tropolone (61%), reveals the possible involvement of NADPH-oxidases, peroxidases and probably phenoloxidases, respectively, in the generation of superoxide anion. Similarly, decrease in nitric oxide generation in the presence of l-NIO (47%) during phagocytosis lends support to the role of nitric oxide generation during cellular immune processes. These findings thus suggest a role for superoxide anion and nitric oxide in the innate defense mechanism, namely phagocytosis, in Macrobrachium rosenbergii.
Authors:
Thiagarajan Raman; Munusamy Arumugam; Periasamy Mullainadhan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-28
Journal Detail:
Title:  Fish & shellfish immunology     Volume:  24     ISSN:  1050-4648     ISO Abbreviation:  Fish Shellfish Immunol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-19     Completed Date:  2008-07-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9505220     Medline TA:  Fish Shellfish Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  337-45     Citation Subset:  IM    
Affiliation:
Laboratory of Pathobiology, Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India. thiagi200@yahoo.co.in <thiagi200@yahoo.co.in>
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MeSH Terms
Descriptor/Qualifier:
Agglutinins / immunology,  metabolism*
Animals
Carbohydrates / pharmacology
Erythrocytes / cytology,  drug effects,  metabolism
Hemagglutination
Hemocytes / immunology,  metabolism*
Humans
Nitric Oxide / metabolism*
Palaemonidae / cytology,  metabolism*
Phagocytosis* / drug effects
Superoxides / metabolism*
Chemical
Reg. No./Substance:
0/Agglutinins; 0/Carbohydrates; 10102-43-9/Nitric Oxide; 11062-77-4/Superoxides

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