| Ageing mouse kidney--not always the SAME old story. | |
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MedLine Citation:
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PMID: 19474281 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: As interest in the ageing kidney grows rapidly, more experimental ageing studies are conducted in the field. One of the main obstacles that researchers have to face is that studies in old animals are often less reproducible than in young animals. We have observed that the aged animal's provenance can be an overlooked factor accounting for such experimental heterogeneity. METHODS: Male C57BL/6J mice aged 19-22 months were purchased from four different suppliers. Baseline renal parameters were evaluated by measuring serum urea, serum creatinine and proteinuria. Renal morphology was analysed by quantifying glomerulosclerosis, interstitial fibrosis and amyloid deposits on paraffin sections stained with PAS, Masson trichrome, Sirius red and Congo red. RESULTS: We found normal renal ageing in mice from three sources, but an unexpected renal pathology in mice from one major European supplier. Mice from this supplier had significantly elevated serum urea, creatinine values and an increased urinary protein excretion. Corresponding kidneys displayed massive glomerulosclerosis with evidence of amyloid deposits and increased interstitial fibrosis. CONCLUSIONS: Supplier-dependent differences, such as observed here, can explain irreproducibility of experimental results in renal ageing research. This can be avoided by careful baseline analysis prior to in vivo experiments. |
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Authors:
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Roland Schmitt; Christoph Jacobi; Nathan Susnik; Verena Broecker; Hermann Haller; Anette Melk |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-27 |
Journal Detail:
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Title: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association Volume: 24 ISSN: 1460-2385 ISO Abbreviation: Nephrol. Dial. Transplant. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-22 Completed Date: 2009-12-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8706402 Medline TA: Nephrol Dial Transplant Country: England |
Other Details:
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Languages: eng Pagination: 3002-5 Citation Subset: IM |
Affiliation:
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Department of Nephrology, Hannover Medical School, Hannover, Germany. schmitt.roland@mh-hannover.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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physiology* Animals Kidney / anatomy & histology, physiology* Male Mice Mice, Inbred C57BL |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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