| Ageing affects nitric oxide synthase, cyclooxygenase and oxidative stress enzymes expression differently in mesenteric resistance arteries. | |
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MedLine Citation:
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PMID: 16176446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1 Our aim was to study the role of nitric oxide (NO) and arachidonic acid pathways in the alpha(1)-adrenoceptor-mediated vasoconstriction in mesenteric resistance arteries from 3--4 and 22 to 23-month-old Sprague-Dawley rats. 2 The expression of NO synthase (NOS), cyclooxygenase (COX) isoforms, soluble guanylate cyclase, superoxide dismutase and the NAD(P)H oxidase subunits p22(phox) and p 47(phox) were determined. 3 The N(G)-nitro-l-arginine methyl ester, a non-selective NOS inhibitor, shifted to the left but indomethacin and NS 398, non-selective and selective COX-2 inhibitors, shifted to the right the concentration-response curve for the vasoconstriction by phenylephrine in both age groups. 4 Ageing up-regulated endothelial NOS and p22(phox) expression but did not modify COX, soluble guanylate cyclase, superoxide dismutase and p 47(phox) expression. 5 These data suggest that the observed enhancement of eNOS protein expression could constitute a compensatory mechanism to counter-regulate a chronic loss of NO possibly through increased superoxide anion production from NAD(P)H oxidase induced by age. |
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Authors:
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A M Briones; N Montoya; J Giraldo; E Vila |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Autonomic & autacoid pharmacology Volume: 25 ISSN: 1474-8665 ISO Abbreviation: Auton Autacoid Pharmacol Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-09-22 Completed Date: 2006-10-24 Revised Date: 2008-01-22 |
Medline Journal Info:
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Nlm Unique ID: 101157306 Medline TA: Auton Autacoid Pharmacol Country: England |
Other Details:
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Languages: eng Pagination: 155-62 Citation Subset: IM |
Affiliation:
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Departament de Farmacologia, de Terapèutica i de Toxicologia, Facultat de Medicine, Institute de Neuroxiencies, Universitat Automona de Barcelona, 08193 Bellaterra, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine Aging / metabolism* Animals Cyclooxygenase Inhibitors / pharmacology Dose-Response Relationship, Drug Drug Interactions Indomethacin / pharmacology Male Mesenteric Arteries / drug effects, metabolism* NADPH Oxidase / metabolism NG-Nitroarginine Methyl Ester / pharmacology Nitrobenzenes / pharmacology Oxidative Stress* Phenylephrine Rats Rats, Sprague-Dawley Sulfonamides / pharmacology Up-Regulation Vascular Resistance / drug effects Vasoconstrictor Agents Vasodilator Agents |
| Chemical | |
Reg. No./Substance:
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0/Cyclooxygenase Inhibitors; 0/Nitrobenzenes; 0/Sulfonamides; 0/Vasoconstrictor Agents; 0/Vasodilator Agents; 123653-11-2/N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; 50903-99-6/NG-Nitroarginine Methyl Ester; 51-84-3/Acetylcholine; 53-86-1/Indomethacin; 59-42-7/Phenylephrine; EC 1.6.3.1/NADPH Oxidase |
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