Document Detail

Ageing affects anoxia/reoxygenation injury in rat hepatocytes.
MedLine Citation:
PMID:  9829368     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The reoxygenation phase after a period of anoxia leads to oxyradical formation, responsible for damage to cell membranes. Ageing is associated with functional and structural changes in liver cells, which modify their sensitivity to reoxygenation injury. The aim of this study was to determine the effects of ageing on the sensitivity of hepatocytes to anoxia/reoxygenation. METHODS: Oxyradical formation and cell injury were evaluated in hepatocytes isolated from rats of different ages exposed to 2 h of anoxia and 1 h of reoxygenation. Anion superoxide was measured by lucigenin-enhanced chemiluminescence, hydrogen peroxide by luminol-enhanced chemiluminescence, and cell damage by lactate dehydrogenase (LDH) release. RESULTS: During anoxia, oxyradical production dropped to background levels in both groups. LDH release was significantly greater in ageing hepatocytes. During reoxygenation, a massive generation of anion superoxide and hydrogen peroxide, followed by a sharp increase in LDH release, was observed in both groups. However, both oxyradicals and cell injury were significantly greater in liver cells obtained from ageing rats. CONCLUSIONS: The data confirm that hepatocytes produce high levels of free radicals during post-ischemic reoxygenation and suggest that ageing cells are more sensitive to reperfusion injury.
A Gasbarrini; M Simoncini; C Di Campli; S De Notariis; A Colantoni; P Pola; M Bernardi; G Gasbarrini
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of gastroenterology     Volume:  33     ISSN:  0036-5521     ISO Abbreviation:  Scand. J. Gastroenterol.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1999-01-15     Completed Date:  1999-01-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060105     Medline TA:  Scand J Gastroenterol     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  1107-12     Citation Subset:  IM    
Internal Medicine Dept., Catholic University of Rome, Italy.
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MeSH Terms
Aging / pathology*
Cell Hypoxia
Chemiluminescent Measurements
Free Radicals
L-Lactate Dehydrogenase / metabolism
Liver / metabolism,  pathology*
Oxygen / pharmacology*
Rats, Sprague-Dawley
Superoxides / metabolism*
Reg. No./Substance:
0/Free Radicals; 11062-77-4/Superoxides; 7782-44-7/Oxygen; EC Dehydrogenase
Comment In:
Scand J Gastroenterol. 1999 Jul;34(7):735-6   [PMID:  10466887 ]

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