Document Detail


Aged male and female spontaneously hypertensive rats benefit from n-3 polyunsaturated fatty acids supplementation.
MedLine Citation:
PMID:  18373394     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hypertension-induced myocardial metabolic, structural and electrophysiological remodeling deteriorates with aging and contributes to both heart failure and occurrence of malignant arrhythmias. It has been shown in clinical trials that n-3 polyunsaturated fatty acids (n-3 PUFA) reduce the incidence of cardiovascular diseases and sudden cardiac death. We investigated the cardioprotective effects of n-3 PUFA in aged spontaneously hypertensive rats (SHR) and possible cellular mechanisms involved. Male and female 14-moth-old SHR were fed with n-3 PUFA (Vesteralens, Norway, 20 mg/day for two months) and compared with untreated SHR. Results showed that n-3 PUFA supplementation led to 1) significant decline of blood pressure; 2) suppression of inducible ventricular fibrillation (VF) by 57 % (male) and 67 % (female), although the arrhythmogenic substrates, like fibrosis, hypertrophy and abnormal gap junctions distribution were not eliminated; 3) preservation of the cardiomyocytes and the integrity of their junctions; 4) enhancement of energetic metabolism enzyme activity; 5) augmentation of capillary density associated with increased alkaline phosphatase and decreased dipeptidyl peptidase-4 (DPP4) activity and 6/ increase in gap junction channel connexin-43 expression. Thus, aged male as well as female SHR benefit from n-3 PUFA supplementation that results in decrease in VF susceptibility, partly due to an improvement of myocardial metabolic state, cardiomyocyte and cell-to-cell junctions integrity and Cx43 up-regulation.
Authors:
M Mitasíková; S Smidová; A Macsaliová; V Knezl; K Dlugosová; L Okruhlicová; P Weismann; N Tribulová
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-28
Journal Detail:
Title:  Physiological research / Academia Scientiarum Bohemoslovaca     Volume:  57 Suppl 2     ISSN:  0862-8408     ISO Abbreviation:  Physiol Res     Publication Date:  2008  
Date Detail:
Created Date:  2008-08-27     Completed Date:  2008-10-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9112413     Medline TA:  Physiol Res     Country:  Czech Republic    
Other Details:
Languages:  eng     Pagination:  S39-48     Citation Subset:  IM    
Affiliation:
Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Blood Pressure / drug effects
Capillaries / drug effects
Connexin 43 / metabolism
Dietary Supplements*
Disease Models, Animal
Energy Metabolism / drug effects
Fatty Acids, Omega-3 / pharmacology*
Female
Hypertension / complications,  drug therapy*,  metabolism,  physiopathology
Male
Myocardium / enzymology,  metabolism*,  pathology
Myocytes, Cardiac / drug effects,  metabolism
Rats
Rats, Inbred SHR
Tight Junctions / drug effects,  metabolism
Ventricular Fibrillation / etiology,  metabolism,  physiopathology,  prevention & control*
Chemical
Reg. No./Substance:
0/Connexin 43; 0/Fatty Acids, Omega-3

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