| Aged garlic extract inhibits CD36 expression in human macrophages via modulation of the PPARgamma pathway. | |
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MedLine Citation:
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PMID: 20091745 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Expression of CD36 scavenger receptors on macrophages is involved in oxidized low-density lipoprotein uptake and foam cell formation during atherosclerotic lesion development. We examined the effects of aged garlic extract (AGE), a garlic preparation enriched in water-soluble cysteinyl moieties that increases cellular total thiols and glutathione concentrations, on CD36 expression in human monocytes/macrophages (THP-1 cells and primary human monocytes). Compared to control, AGE (1-5 mg/mL) dose-dependently and significantly suppressed CD36 expression up to by 61.8 +/- 7.4% in THP-1-derived macrophages and up to 50.5 +/- 7.1% in primary human macrophages, respectively. Furthermore, AGE prevented induction of CD36 expression by the peroxisome proliferator activator receptor (PPAR) gamma agonist troglitazone, and decreased binding of nuclear proteins to a PPARgamma response element. AGE showed a stronger inhibitory effect on CD36 expression in THP-1 cells during simultaneous incubation with phorbol 12-myristate 13-acetate (PMA) compared to cells that had been pre-incubated with PMA. Furthermore, AGE decreased CD11b expression in a dose-dependent manner. These data indicate that AGE inhibits CD36 expression by modulating the PPARgamma pathway in human macrophages and monocytes differentiation into macrophages, and suggests that the extract could be useful for the prevention of atherosclerotic lesions. |
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Authors:
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Naoaki Morihara; Nagatoshi Ide; Norbert Weiss |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Phytotherapy research : PTR Volume: 24 ISSN: 1099-1573 ISO Abbreviation: Phytother Res Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-30 Completed Date: 2010-06-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8904486 Medline TA: Phytother Res Country: England |
Other Details:
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Languages: eng Pagination: 602-8 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 John Wiley & Sons, Ltd. |
Affiliation:
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Center for Vascular Medicine, Section Angiology, Medical Policlinic, University of Munich Medical Center, Pettenkoferstrasse 8A, D-80336 Munich, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD36
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metabolism* Atherosclerosis / prevention & control* Cell Differentiation / drug effects Cell Line, Tumor Drug Evaluation, Preclinical Garlic* Humans Macrophages / cytology, drug effects*, metabolism Monocytes / drug effects PPAR gamma / metabolism Phytotherapy Plant Extracts / pharmacology*, therapeutic use |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD36; 0/PPAR gamma; 0/Plant Extracts |
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