| Aged garlic extract induces proliferation and ameliorates gentamicin-induced toxicity in LLC-PK1 cells. | |
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MedLine Citation:
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PMID: 16397848 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Gentamicin (GM)-induced nephrotoxicity limits the use of this antibiotic. It has been shown that aged garlic extract (AGE) and S-allylcysteine (SAC), the most abundant organosulfur compound in AGE, ameliorate GM-induced nephrotoxicity in rats. The present communication evaluated the effect of AGE and SAC on proliferation and on GM-induced toxicity and genotoxicity of porcine kidney epithelial cell line (LLC-PK1 cells). The cells were preincubated with different concentrations of AGE or SAC for 12 h before incubation with 8 mm GM for an additional 72 h. At the end of this time, cell viability, genotoxicity and proliferation were evaluated. AGE stimulated cell proliferation and protected LLC-PK1 cells from GM-mediated toxicity and genotoxicity. SAC partially prevented only GM-induced genotoxicity. These results suggest that the stimulation of cell proliferation could possibly be one of the mechanisms involved in the in vitro protective effect of AGE in GM-induced toxicity of LLC-PK1 cells. |
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Authors:
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Marco A Velasco-Velázquez; Perla D Maldonado; Diana Barrera; Víctor Torres; Alejandro Zentella-Dehesa; José Pedraza-Chaverrí |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Phytotherapy research : PTR Volume: 20 ISSN: 0951-418X ISO Abbreviation: Phytother Res Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-01-11 Completed Date: 2006-09-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8904486 Medline TA: Phytother Res Country: England |
Other Details:
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Languages: eng Pagination: 76-8 Citation Subset: IM |
Copyright Information:
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Copyright 2006 John Wiley & Sons, Ltd. |
Affiliation:
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Departamento de Farmacología, Facultad de Medicina, UNAM, Mexico City, Mexico. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Anti-Bacterial Agents / toxicity* Bromodeoxyuridine / metabolism Cell Proliferation / drug effects Cysteine / analogs & derivatives*, pharmacology Dose-Response Relationship, Drug Drug Interactions Enzyme-Linked Immunosorbent Assay Garlic* / chemistry Gentamicins / toxicity* LLC-PK1 Cells Mutagenicity Tests Phytotherapy Plant Extracts / pharmacology* Protective Agents / pharmacology* Toxicity Tests |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Gentamicins; 0/Plant Extracts; 0/Protective Agents; 21593-77-1/S-allylcysteine; 52-90-4/Cysteine; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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