| Age and vitamin E-induced changes in gene expression profiles of T cells. | |
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MedLine Citation:
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PMID: 17056531 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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T cells are vulnerable to age-associated changes. Vitamin E has been shown to improve T cell functions in the old. We studied gene expression profiles of T cells to better understand the underlying mechanisms of age and vitamin E-induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (supplemented) ppm of vitamin E for 4 wks. Gene expression profiles of T cells were assessed using microarray analysis with/without anti-CD3/anti-CD28 stimulation. Genes associated with cytokines/chemokines, transcriptional regulation, signal transduction, cell cycle, and apoptosis were significantly up-regulated upon stimulation. Higher SOCS3 and lower growth factor independent 1 (Gfi-1) expression in old T cells may contribute to age-associated decline in proliferation. Higher Gadd45 and lower Bcl2 expression may contribute to increased apoptosis in old T cells. Vitamin E supplementation resulted in higher expression of genes involved in cell cycle regulation (Ccnb2, Cdc2, Cdc6) in old T cells. Vitamin E supplementation resulted in higher up-regulation of IL-2 expression in young and old T cells and lower up-regulation of IL-4 expression in old T cells following stimulation. These findings suggest that aging has significant effects on the expression of genes associated with signal transduction, transcriptional regulation, and apoptosis pathways in T cells, and vitamin E has a significant impact on the expression of genes associated with cell cycle and Th1/Th2 balance in old T cells. Further studies are needed to determine whether these changes are due to the effects of aging at a single-cell level or to the shift in the ratio of naïve:memory T cells with age. |
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Authors:
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Sung Nim Han; Oskar Adolfsson; Cheol-Koo Lee; Tomas A Prolla; Jose Ordovas; Simin Nikbin Meydani |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 177 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-10-23 Completed Date: 2006-12-13 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 6052-61 Citation Subset: AIM; IM |
Affiliation:
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Nutritional Immunology Laboratory, Jean Mayer U.S. Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, 711 Washington Street, Boston, MA 02111, USA. sungnim.han@tufts.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Aging / genetics, immunology* Animals Apoptosis / genetics Cell Cycle / genetics Gene Expression / drug effects Gene Expression Profiling* Genes, Immunoglobulin Lymphocyte Activation / genetics Male Mice Mice, Inbred C57BL Receptors, Antigen, T-Cell / genetics T-Lymphocytes / drug effects*, metabolism* Vitamin E / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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2R01 AG 009140-10A1/AG/NIA NIH HHS; P30 DK040561-11/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Antigen, T-Cell; 1406-18-4/Vitamin E |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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