Document Detail


Age and vitamin E-induced changes in gene expression profiles of T cells.
MedLine Citation:
PMID:  17056531     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
T cells are vulnerable to age-associated changes. Vitamin E has been shown to improve T cell functions in the old. We studied gene expression profiles of T cells to better understand the underlying mechanisms of age and vitamin E-induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (supplemented) ppm of vitamin E for 4 wks. Gene expression profiles of T cells were assessed using microarray analysis with/without anti-CD3/anti-CD28 stimulation. Genes associated with cytokines/chemokines, transcriptional regulation, signal transduction, cell cycle, and apoptosis were significantly up-regulated upon stimulation. Higher SOCS3 and lower growth factor independent 1 (Gfi-1) expression in old T cells may contribute to age-associated decline in proliferation. Higher Gadd45 and lower Bcl2 expression may contribute to increased apoptosis in old T cells. Vitamin E supplementation resulted in higher expression of genes involved in cell cycle regulation (Ccnb2, Cdc2, Cdc6) in old T cells. Vitamin E supplementation resulted in higher up-regulation of IL-2 expression in young and old T cells and lower up-regulation of IL-4 expression in old T cells following stimulation. These findings suggest that aging has significant effects on the expression of genes associated with signal transduction, transcriptional regulation, and apoptosis pathways in T cells, and vitamin E has a significant impact on the expression of genes associated with cell cycle and Th1/Th2 balance in old T cells. Further studies are needed to determine whether these changes are due to the effects of aging at a single-cell level or to the shift in the ratio of naïve:memory T cells with age.
Authors:
Sung Nim Han; Oskar Adolfsson; Cheol-Koo Lee; Tomas A Prolla; Jose Ordovas; Simin Nikbin Meydani
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  177     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-23     Completed Date:  2006-12-13     Revised Date:  2011-11-01    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6052-61     Citation Subset:  AIM; IM    
Affiliation:
Nutritional Immunology Laboratory, Jean Mayer U.S. Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, 711 Washington Street, Boston, MA 02111, USA. sungnim.han@tufts.edu
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Aging / genetics,  immunology*
Animals
Apoptosis / genetics
Cell Cycle / genetics
Gene Expression / drug effects
Gene Expression Profiling*
Genes, Immunoglobulin
Lymphocyte Activation / genetics
Male
Mice
Mice, Inbred C57BL
Receptors, Antigen, T-Cell / genetics
T-Lymphocytes / drug effects*,  metabolism*
Vitamin E / pharmacology*
Grant Support
ID/Acronym/Agency:
2R01 AG 009140-10A1/AG/NIA NIH HHS; P30 DK040561-11/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Antigen, T-Cell; 1406-18-4/Vitamin E

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