Document Detail


Age-related proteomic changes in the subventricular zone and their association with neural stem/progenitor cell proliferation.
MedLine Citation:
PMID:  22344963     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the mammalian central nervous system, generation of new neurons persists in the subventricular zone (SVZ) throughout life. However, the capacity for neurogenesis in this region declines with aging. Recent studies have examined the degree of these age-related neurogenic declines and the changes of cytoarchitecture of the SVZ with aging. However, little is known about the molecular changes in the SVZ with aging. In this study, we dissected the SVZs from rats aged postnatal day 28, 3 months, and 24 months. The SVZ tissues were processed for 2-D gel electrophoresis to identify protein changes following aging. Protein spots were subsequently subjected to mass spectrometry analysis to compare age-related alterations in the SVZ proteome. We also examined the level of cell proliferation in the SVZ in animals of these three age groups by using bromodeoxyuridine labeling. We found significant age-related changes in the expression of several proteins that play critical roles in the proliferation and survival of neural stem/progenitor cells in the SVZ. Among these proteins, glial fibrillary acidic protein, ubiquitin carboxy terminal hydrolase 1, glutathione S-transferase omega, and preproalbumin were increased with aging, whereas collapsin response-mediated protein 4 (CRMP-4), CRMP-5, and microsomal protease ER60 exhibited declines with aging. We have also observed a significant decline of neural stem/progenitor cell proliferation in the SVZ with aging. These alterations in protein expression in the SVZ with aging likely underlie the diminishing proliferative capacity of stem/progenitor cells in the aging brain.
Authors:
Melissa J McGinn; Raymond J Colello; Dong Sun
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-02-16
Journal Detail:
Title:  Journal of neuroscience research     Volume:  90     ISSN:  1097-4547     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-04-10     Completed Date:  2012-08-10     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1159-68     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Aging / physiology*
Animals
Animals, Newborn
Bromodeoxyuridine / metabolism
Cell Proliferation*
Cerebral Ventricles* / cytology,  growth & development,  metabolism
Electrophoresis, Gel, Two-Dimensional
Gene Expression Regulation, Developmental / physiology
Glial Fibrillary Acidic Protein / metabolism
Intermediate Filament Proteins / metabolism
Male
Nerve Tissue Proteins / metabolism
Nestin
Neural Stem Cells / physiology*
Proteomics*
Rats
Rats, Sprague-Dawley
Spectrometry, Mass, Electrospray Ionization / methods
Grant Support
ID/Acronym/Agency:
5P30NS047463/NS/NINDS NIH HHS; NS048377/NS/NINDS NIH HHS; NS055086/NS/NINDS NIH HHS; R01 NS055086/NS/NINDS NIH HHS; R01 NS055086-01A2/NS/NINDS NIH HHS; R21 NS048377-02/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/Nes protein, rat; 0/Nestin; G34N38R2N1/Bromodeoxyuridine
Comments/Corrections

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