Document Detail

Age-related lung cell response to urban Buenos Aires air particle soluble fraction.
MedLine Citation:
PMID:  18313661     Owner:  NLM     Status:  MEDLINE    
Exposure to particulate matter (PM) may alter lung homeostasis inducing changes in fluid balance and host defense. Bioavailability of soluble PM compounds like polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and transition metals has been shown to play a key role in lung injury. We have previously characterized the size, shape, and chemical components of urban air particles from Buenos Aires (UAP-BA) and their biological impact on lungs. Herein, we evaluate the possible toxic effect of UAP-BA-soluble fraction (UAP-BAsf) on pulmonary cells obtained from young (1-2 months old) and aged (9-12 months old) Wistar rats using phagocytosis, oxidant-antioxidant generation, and apoptosis as endpoints. UAP-BA were collected in downtown BA and residual oil fly ash (ROFA), employed as a positive control, was collected from Boston Edison Co., Mystic Power Plant, Mystic, CT, USA. Both particle-soluble fractions (sf) were employed at concentrations ranging from 0 to 100 microg/mL. UAP-BAsf and ROFAsf even at the lowest dose assayed (10 microg/mL) showed in both lung cell populations the ability to stimulate phagocytosis and increase superoxide anion (O(2)(-)) generation. Both types of air particles caused a marked intracellular oxidant stress in aged pulmonary cells that may contribute to subsequent cell activation and production of proinflammatory mediators, leading to cell dysfunction. These data suggest that the impact of UAP-BAsf on phagocytosis, oxidant radical generation, and apoptosis is clearly dependent on the maturational state of the animal and might have different mechanisms of action.
Agustín Ostachuk; Pablo Evelson; Susana Martin; Laura Dawidowski; J Sebastián Yakisich; Deborah R Tasat
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-03
Journal Detail:
Title:  Environmental research     Volume:  107     ISSN:  1096-0953     ISO Abbreviation:  Environ. Res.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-13     Completed Date:  2008-07-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0147621     Medline TA:  Environ Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  170-7     Citation Subset:  IM    
School of Science and Technology, National University of General Martín, Martín de Irigoyen 3100, 1650 San Martín, Buenos Aires, Argentina.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Age Factors
Air Pollution / adverse effects*
Antioxidants / metabolism
Apoptosis / drug effects
Bronchoalveolar Lavage Fluid / cytology
Cell Survival
Cells, Cultured
Lung / drug effects*,  metabolism
Macrophages, Alveolar / drug effects*
Particulate Matter / toxicity*
Phagocytosis / drug effects
Rats, Wistar
Superoxides / metabolism
Reg. No./Substance:
0/Antioxidants; 0/Particulate Matter; 11062-77-4/Superoxides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Relationship between insulin sensitivity and exposure to dioxins and polychlorinated biphenyls in pr...
Next Document:  Nitric oxide down-regulates the expression of organic cation transporters (OCT) 1 and 2 in rat kidne...