| Age-related differences in the metabolism of sulphite to sulphate and in the identification of sulphur trioxide radical in human polymorphonuclear leukocytes. | |
| | |
MedLine Citation:
|
PMID: 8803926 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Sulphite oxidation and sulphur trioxide radical formation were studied in polymorphonuclear leukocytes (PMNs) isolated from healthy young, old and centenarian donors and from patients with Down's syndrome. The sulphur radical formation measured by electron spin resonance spectroscopy-spin trapping (EPR-ST) was correlated with the activity of sulphite oxidase and with the rate of sulphite oxidation to sulphate by PMNs. Sulphite metabolism was studied both in resting, and phorbol myristate acetate (PMA) stimulated freshly isolated cells. The rate of sulphur trioxide radical formation was demonstrated by use of the spin trapping agent 5,5-dimethyl-1-pyroline-1-oxide (DMPO) with subsequent formation of an adduct. The intensity of adduct formation was most intense in cells with low sulphite oxidase activity, while a mixture of the adduct and of DMPO hydroxyl radical was mainly observed in cells with high sulphite oxidase activity. Furthermore, experiments carried out on purified sulphite oxidase showed that in the presence of sulphite the enzyme could also give rise to a DMPO-OH adduct. Sulphite oxidase activity in cells isolated from healthy young and old donors was positive correlated with both rates of sulphur trioxide radical formation and sulphite oxidation to sulphate, respectively. However, sulphite oxidase activity in cells isolated from centenarians and patients with Down's syndrome seems to loose partly its rate of oxidising sulphite to sulphate. The intensity of the sulphur centred radical adduct increased in the two latter groups of population and the radical observed was predominantly sulphur trioxide radical. |
| | |
Authors:
|
D Constantin; A Bini; E Meletti; P Moldeus; D Monti; A Tomasi |
Related Documents
:
|
19915836 - Radiobiological effects of hypoxia-dependent uptake of 64cu-atsm: enhanced dna damage a... 2690676 - A device for aeration and mixing of cell and organelle suspensions during nuclear magne... 8043316 - Interaction of nitrobenzoates with haemoglobin in red blood cells and a haemolysate. 16817596 - Metabolic effects of fructose 1,6-bisphosphate in normoxic and hypoxic states of mg63 o... 3885026 - Activation of n-acetoxy- and n-hydroxy-2-acetylaminofluorene to mutagenic and cytotoxic... 19446606 - A novel wd-40 repeat protein wdr26 suppresses h2o2-induced cell death in neural cells. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Mechanisms of ageing and development Volume: 88 ISSN: 0047-6374 ISO Abbreviation: Mech. Ageing Dev. Publication Date: 1996 Jul |
Date Detail:
|
Created Date: 1996-12-16 Completed Date: 1996-12-16 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0347227 Medline TA: Mech Ageing Dev Country: IRELAND |
Other Details:
|
Languages: eng Pagination: 95-109 Citation Subset: IM |
Affiliation:
|
Medical Research Council, Toxicology Unit, University of Leicester, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aging
/
metabolism* Cyclic N-Oxides / pharmacology Electron Spin Resonance Spectroscopy Free Radicals Humans Neutrophils / metabolism* Oxidation-Reduction Oxidoreductases Acting on Sulfur Group Donors / metabolism Oxygen Consumption Spin Labels Spin Trapping Sulfates / metabolism* Sulfites / metabolism* Sulfur Oxides / metabolism* Tetradecanoylphorbol Acetate / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Cyclic N-Oxides; 0/Free Radicals; 0/Spin Labels; 0/Sulfates; 0/Sulfites; 0/Sulfur Oxides; 16561-29-8/Tetradecanoylphorbol Acetate; 3317-61-1/5,5-dimethyl-1-pyrroline-1-oxide; 7446-11-9/sulfur trioxide; EC 1.8.-/Oxidoreductases Acting on Sulfur Group Donors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nuclear degeneration in epidermal cells of drying onion bulb leaf bases.
Next Document: Alterations in transcription factor-binding activities to fibronectin promoter during aging of vascu...