Document Detail


Age-related cochlear cytokine gene expression in the BALB/cJ mouse with systemic versus intratympanic dosing of steroid drugs.
MedLine Citation:
PMID:  23768053     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CONCLUSION: Age-related differences in the expression of inflammatory cytokines in the inner ear may contribute to the development of age-related hearing loss (ARHL).
OBJECTIVES: ARHL is characterized by tissue remodeling, ischemia, ion homeostasis, and inflammation. Steroid therapy is an otoprotective strategy that likely acts by reducing inflammation. We examined age-related changes in cytokine gene expression in the cochlea of the BALB/cJ mouse model of premature ARHL after systemic or intratympanic steroid delivery.
METHODS: 'Young' (2.5-3 months) and 'Old' (5-9 months) mice were treated with dexamethasone or fludrocortisone administered either orally or intratympanically. Cytokine gene expression in cochlear RNA was analyzed using prefabricated cDNA arrays. Old groups were compared to Young groups to identify age-related changes.
RESULTS: Down-regulation of a cytokine associated with bone remodeling (SPP1) was observed in the untreated Old group. Numerous genes were up- or down-regulated by more than twofold by steroid treatment, including proinflammatory interleukins (IL-16) and anti-inflammatory cytokines.
Authors:
Sara A Tokarz; Jiaqing Pang; Anna Grosz; J Beth Kempton; Dennis R Trune; De-Ann M Pillers
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta oto-laryngologica     Volume:  133     ISSN:  1651-2251     ISO Abbreviation:  Acta Otolaryngol.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-17     Completed Date:  2013-10-23     Revised Date:  2014-03-31    
Medline Journal Info:
Nlm Unique ID:  0370354     Medline TA:  Acta Otolaryngol     Country:  England    
Other Details:
Languages:  eng     Pagination:  685-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Age Factors
Animals
Cytokines / genetics*,  metabolism
Dexamethasone / pharmacology*
Dose-Response Relationship, Drug
Down-Regulation
Fludrocortisone / pharmacology*
Gene Expression Regulation / drug effects*
Injections, Intralesional
Instillation, Drug
Male
Mice
Mice, Inbred BALB C
Models, Animal
Oligonucleotide Array Sequence Analysis
Random Allocation
Reference Values
Risk Assessment
Sensitivity and Specificity
Tympanic Membrane / drug effects*
Grant Support
ID/Acronym/Agency:
R01 DC005593/DC/NIDCD NIH HHS; R01 DC05593/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/Cytokines; 7S5I7G3JQL/Dexamethasone; U0476M545B/Fludrocortisone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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