Document Detail


Age-related changes in mesenchymal stem cells derived from rhesus macaque bone marrow.
MedLine Citation:
PMID:  20969724     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The regeneration potential of mesenchymal stem cells (MSCs) diminishes with advanced age and this diminished potential is associated with changes in cellular functions. This study compared MSCs isolated from the bone marrow of rhesus monkeys (rBMSCs) in three age groups: young (< 5 years), middle (8-10 years), and old (> 12 years). The effects of aging on stem cell properties and indicators of stem cell fitness such as proliferation, differentiation, circadian rhythms, stress response proteins, miRNA expression, and global histone modifications in rBMSCs were analyzed. rBMSCs demonstrated decreased capacities for proliferation and differentiation as a function of age. The production of heat shock protein 70 (HSP70) and heat shock factor 1 (HSF1) were also reduced with increasing age. The level of a core circadian protein, Rev-erb α, was significantly increased in rBMSCs from old animals. Furthermore, analysis of miRNA expression profiles revealed an up-regulation of mir-766 and mir-558 and a down-regulation of mir-let-7f, mir-125b, mir-222, mir-199-3p, mir-23a, and mir-221 in old rBMSCs compare to young rBMSCs. However, there were no significant age-related changes in the global histone modification profiles of the four histone core proteins: H2A, H2B, H3, and H4 on rBMSCs. These changes represent novel insights into the aging process and could have implications regarding the potential for autologous stem cells therapy in older patients.
Authors:
Ji Min Yu; Xiying Wu; Jeffrey M Gimble; Xiaoyan Guan; Michael A Freitas; Bruce A Bunnell
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Aging cell     Volume:  10     ISSN:  1474-9726     ISO Abbreviation:  Aging Cell     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-12     Completed Date:  2011-07-11     Revised Date:  2014-03-21    
Medline Journal Info:
Nlm Unique ID:  101130839     Medline TA:  Aging Cell     Country:  England    
Other Details:
Languages:  eng     Pagination:  66-79     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Aging / genetics,  metabolism*
Animals
Bone Marrow / physiology
Bone Marrow Cells / cytology,  physiology
Cell Aging / physiology*
Cell Differentiation / physiology
Cell Proliferation
Cells, Cultured
Circadian Rhythm / physiology
Circadian Rhythm Signaling Peptides and Proteins / genetics,  metabolism
Down-Regulation / physiology
Female
Gene Expression
Heat-Shock Proteins / genetics,  metabolism
Histones / genetics,  metabolism
Macaca mulatta / genetics*,  metabolism
Male
Mesenchymal Stem Cell Transplantation
Mesenchymal Stromal Cells / cytology,  physiology*
MicroRNAs / genetics,  metabolism
Up-Regulation / physiology
Grant Support
ID/Acronym/Agency:
P51 RR000164/RR/NCRR NIH HHS; R01 CA107106/CA/NCI NIH HHS; R01 CA107106-07/CA/NCI NIH HHS; RR00164/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Circadian Rhythm Signaling Peptides and Proteins; 0/Heat-Shock Proteins; 0/Histones; 0/MicroRNAs

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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