| Age-related changes in mesenchymal stem cells derived from rhesus macaque bone marrow. | |
| | |
MedLine Citation:
|
PMID: 20969724 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The regeneration potential of mesenchymal stem cells (MSCs) diminishes with advanced age and this diminished potential is associated with changes in cellular functions. This study compared MSCs isolated from the bone marrow of rhesus monkeys (rBMSCs) in three age groups: young (< 5 years), middle (8-10 years), and old (> 12 years). The effects of aging on stem cell properties and indicators of stem cell fitness such as proliferation, differentiation, circadian rhythms, stress response proteins, miRNA expression, and global histone modifications in rBMSCs were analyzed. rBMSCs demonstrated decreased capacities for proliferation and differentiation as a function of age. The production of heat shock protein 70 (HSP70) and heat shock factor 1 (HSF1) were also reduced with increasing age. The level of a core circadian protein, Rev-erb α, was significantly increased in rBMSCs from old animals. Furthermore, analysis of miRNA expression profiles revealed an up-regulation of mir-766 and mir-558 and a down-regulation of mir-let-7f, mir-125b, mir-222, mir-199-3p, mir-23a, and mir-221 in old rBMSCs compare to young rBMSCs. However, there were no significant age-related changes in the global histone modification profiles of the four histone core proteins: H2A, H2B, H3, and H4 on rBMSCs. These changes represent novel insights into the aging process and could have implications regarding the potential for autologous stem cells therapy in older patients. |
| | |
Authors:
|
Ji Min Yu; Xiying Wu; Jeffrey M Gimble; Xiaoyan Guan; Michael A Freitas; Bruce A Bunnell |
Related Documents
:
|
23046204 - Immunotoxicity and pulmonary toxicity induced by paints in egyptian painters. 15728664 - A central role for ceramide in the age-related acceleration of apoptosis in the female ... 9249684 - Hematological and plasma biochemical reference intervals in young white storks. 23053384 - The distribution of stylohyoid chain anatomic variations by age groups and gender: an a... 8859084 - Prevalence and associations of retinal vein occlusion in australia. the blue mountains ... 9157114 - Handedness and its association with gender-related psychological and physiological char... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Aging cell Volume: 10 ISSN: 1474-9726 ISO Abbreviation: Aging Cell Publication Date: 2011 Feb |
Date Detail:
|
Created Date: 2011-01-12 Completed Date: 2011-07-11 Revised Date: 2013-04-17 |
Medline Journal Info:
|
Nlm Unique ID: 101130839 Medline TA: Aging Cell Country: England |
Other Details:
|
Languages: eng Pagination: 66-79 Citation Subset: IM |
Affiliation:
|
Division of Gene Therapy, Tulane National Primate Research Center, Covington, LA 70433, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Age Factors Aging / genetics, metabolism* Animals Bone Marrow / physiology Bone Marrow Cells / cytology, physiology Cell Aging / physiology* Cell Differentiation / physiology Cell Proliferation Cells, Cultured Circadian Rhythm / physiology Circadian Rhythm Signaling Peptides and Proteins / genetics, metabolism Down-Regulation / physiology Female Gene Expression Heat-Shock Proteins / genetics, metabolism Histones / genetics, metabolism Macaca mulatta / genetics*, metabolism Male Mesenchymal Stem Cell Transplantation Mesenchymal Stromal Cells / cytology, physiology* MicroRNAs / genetics, metabolism Up-Regulation / physiology |
| Grant Support | |
ID/Acronym/Agency:
|
R01 CA107106/CA/NCI NIH HHS; R01 CA107106-07/CA/NCI NIH HHS; RR00164/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Circadian Rhythm Signaling Peptides and Proteins; 0/Heat-Shock Proteins; 0/Histones; 0/MicroRNAs |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Proteolysis of calcineurin is increased in human hippocampus during mild cognitive impairment and is...
Next Document: The effects of clonidine on arterial baroreflex sensitivity and cardiopulmonary barorefex control of...