Document Detail


Age-related changes in glutathione and glutathione-related enzymes in rat brain.
MedLine Citation:
PMID:  16647047     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The most reliable and robust risk factor for some neurodegenerative diseases is aging. It has been proposed that processes of aging are associated with the generation of reactive oxygen species and a disturbance of glutathione homeostasis in the brain. Yet, aged animals have rarely been used to model the diseases that are considered to be age-related such as Parkinson's or Alzheimer's disease. This suggests that the results from these studies would be more valuable if aged animals were used. The present study was designed to provide insight into the glutathione redox state in young and aged rat siblings of both genders by studying the enzyme activities related to glutathione synthesis, cycling, and usage. The results suggested a significant age-related reduction of reduced glutathione (GSH) level in all brain regions examined, associated with an increase of GSH oxidation to glutathione disulfide (GSSG) and decrease of the GSH/GSSG ratio. These changes were accompanied by diminished gamma-glutamylcysteine synthetase activity in de novo glutathione synthesis and increased lipid peroxidation. In addition, these changes were associated with increased enzyme activities related to the GSH usage (glutathione peroxidase, gamma-glutamyl transpeptidase, and glutathione S-transferase). The results indicate that aged animals are likely more vulnerable to oxidative stress and insinuate the roles of aged animals in modeling age-related neurodegeneration diseases.
Authors:
Yuangui Zhu; Paul M Carvey; Zaodung Ling
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-05-02
Journal Detail:
Title:  Brain research     Volume:  1090     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-06-12     Completed Date:  2006-09-25     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  35-44     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Rush University Medical Center, Chicago, IL 60612, USA.
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MeSH Terms
Descriptor/Qualifier:
Aging / physiology*
Animals
Brain / enzymology*
Brain Chemistry / physiology*
Down-Regulation / physiology
Enzymes / metabolism*
Female
Glutamate-Cysteine Ligase / metabolism
Glutathione / metabolism*
Glutathione Peroxidase / metabolism
Glutathione Transferase / metabolism
Male
Neurodegenerative Diseases / enzymology,  physiopathology
Oxidation-Reduction
Oxidative Stress / physiology*
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species / metabolism
Sex Characteristics
gamma-Glutamyltransferase / metabolism
Grant Support
ID/Acronym/Agency:
012307//PHS HHS; NS045316/NS/NINDS NIH HHS; R01 NS045316-01/NS/NINDS NIH HHS; R01 NS045316-02/NS/NINDS NIH HHS; R01 NS045316-03/NS/NINDS NIH HHS; R01 NS045316-04/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Enzymes; 0/Reactive Oxygen Species; 70-18-8/Glutathione; EC 1.11.1.9/Glutathione Peroxidase; EC 2.3.2.2/gamma-Glutamyltransferase; EC 2.5.1.18/Glutathione Transferase; EC 6.3.2.2/Glutamate-Cysteine Ligase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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