| Age-related changes in GAD levels in the central auditory system of the rat. | |
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MedLine Citation:
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PMID: 18930128 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Changes in the levels of gamma-aminobutyric acid (GABA) are known to occur in different parts of the brain during aging. In our study we attempted to define the effect that aging has on glutamate decarboxylase (GAD), the key enzyme in the synthesis of GABA, in the central parts of the auditory system. Age-related changes in GAD65 and GAD67 levels were investigated using immunohistochemistry and Western blotting in the inferior colliculus (IC), the auditory cortex (AC) and the visual cortex in Long-Evans rats. The results show that aging is associated with a decrease in the numbers of GAD65- and 67-immunoreactive neurons and the optical density of their somas in both the IC and AC. Western blot analysis revealed a pronounced age-related decline in the levels of GAD65 and 67 proteins in both the IC and AC. For comparison, in the visual cortex the decrease in both proteins was less pronounced than in the IC and AC. A similar pattern of age-related changes was found in Fischer 344 rats, a strain that manifests a rapid loss of hearing function with aging. The observed age-related decline in the levels of GAD65 and 67 may contribute significantly to the deterioration of hearing function that accompanies aging in mammals, including man. |
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Authors:
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Jana Burianova; Ladislav Ouda; Oliver Profant; Josef Syka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-04 |
Journal Detail:
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Title: Experimental gerontology Volume: 44 ISSN: 1873-6815 ISO Abbreviation: Exp. Gerontol. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-02-16 Completed Date: 2010-02-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0047061 Medline TA: Exp Gerontol Country: England |
Other Details:
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Languages: eng Pagination: 161-9 Citation Subset: IM |
Affiliation:
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Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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metabolism* Analysis of Variance Animals Auditory Cortex / metabolism* Blotting, Western / methods Glutamate Decarboxylase / metabolism* Humans Inferior Colliculi / metabolism* Presbycusis / metabolism* Protein Isoforms / analysis, metabolism Rats Rats, Inbred F344 Rats, Long-Evans Species Specificity gamma-Aminobutyric Acid / metabolism*, physiology |
| Chemical | |
Reg. No./Substance:
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0/Protein Isoforms; 56-12-2/gamma-Aminobutyric Acid; EC 4.1.1.15/Glutamate Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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