Document Detail

Age related changes in 5-methylcytosine content in human peripheral leukocytes and placentas: an HPLC-based study.
MedLine Citation:
PMID:  15180700     Owner:  NLM     Status:  MEDLINE    
The goal of the present study was to investigate inter-individual and age-dependent variation of global DNA methylation in human tissues. In this work, we examined 5-methyldeoxycytidine ((met)C) content by HPLC in human peripheral blood leukocytes obtained from 76 healthy individuals of ages varying from 4 to 94 years (yr), and 39 human placentas from various gestational stages. The HPLC analysis revealed a significant variation of (met)C across individuals and is consistent with the previous findings of age-dependent decrease of global methylation levels in human tissues. The age-dependent decrease of (met)C was relatively small, but statistically highly significant (p= 0.0002) in the aged group (65.9 +/- 8.9 [mean age +/- SD] yr; n = 22) in comparison to the young adult group (19.3 +/- 1.4 yr; n = 21). Males showed a subtle but statistically significant higher mean (met)C content than females. In contrast to the peripheral blood samples, DNA extracted from placentas exhibited gestational stage-dependent increase of methylation levels that appeared to inversely correlate with the expression levels of human endogenous retroviruses. These data may be helpful in further studies of DNA methylation, such as inheritance of epigenetic patterns, environment-induced changes, and involvement of epigenetic changes in disease.
C Fuke; M Shimabukuro; A Petronis; J Sugimoto; T Oda; K Miura; T Miyazaki; C Ogura; Y Okazaki; Y Jinno
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of human genetics     Volume:  68     ISSN:  0003-4800     ISO Abbreviation:  Ann. Hum. Genet.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-06-07     Completed Date:  2004-09-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0416661     Medline TA:  Ann Hum Genet     Country:  England    
Other Details:
Languages:  eng     Pagination:  196-204     Citation Subset:  IM    
Department of Legal Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
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MeSH Terms
Aged, 80 and over
Aging / physiology*
Child, Preschool
Chromatography, High Pressure Liquid
DNA Methylation*
Deoxycytidine / analogs & derivatives*,  blood*
Endogenous Retroviruses / genetics
Leukocytes / metabolism*
Methylenetetrahydrofolate Reductase (NADPH2) / genetics
Middle Aged
Placenta / metabolism*
Pregnancy Trimesters
Reg. No./Substance:
838-07-3/5-methyldeoxycytidine; 951-77-9/Deoxycytidine; EC Reductase (NADPH2)
Erratum In:
Ann Hum Genet. 2005 Jan;69(Pt 1):134

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