Document Detail

Age-related changes of cell outgrowth from rat calvarial and mandibular bone in vitro.
MedLine Citation:
PMID:  17055735     Owner:  NLM     Status:  MEDLINE    
AIM: To establish whether within an autograft ageing has an impact on the number of cells capable of responding to growth and differentiation factors released at defect sites. MATERIAL AND METHODS: The number of cells that grow out from calvarial and mandibular explants of rats 6 weeks and 9 months of age was evaluated and their response to mitogenic, chemotactic, and differentiation factors was characterized in vitro. The cell number was determined by automated counting, proliferation was evaluated by measuring the amount of (tritiated) (3)[H]thymidine incorporated into the DNA, and migration was assessed with the modified Boyden chamber assay. Alkaline phosphatase activity served as a marker of osteogenic differentiation. RESULTS: A confluent layer of cells was observed in mandibular and calvarial explant cultures derived from young rats within 10 days, while only sporadic cells were counted in cultures from adult animals. The number of cells derived from calvarial and mandibular bone of young rats was nine and five times higher, respectively, than that in explants from adult rats. Cells isolated from calvarial and mandibular explants responded to platelet-released supernatant by increased proliferation and migration in both age groups. Upon incubation with bone morphogenetic protein (BMP-6), the alkaline phosphatase activity of calvaria-derived cells from both age groups was higher than in unstimulated controls. Only one sample of cells derived from mandibular explants showed responsiveness to BMP-6 in the young age group, whereas none of the cell samples responded to BMP-6 in the adult age group. CONCLUSION: The number of cells capable of growing out from mandibular and calvarial grafts in vitro is lower in adult animals than in young animals. However, bone-derived cells from both young and adult rats respond to growth and differentiation factors present at defect sites, while there are topographic differences in the responsiveness to BMP-6.
Silvia Cei; Birgit Mair; Barbara Kandler; Mario Gabriele; Georg Watzek; Reinhard Gruber
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Publication Detail:
Type:  Journal Article     Date:  2006-10-20
Journal Detail:
Title:  Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery     Volume:  34     ISSN:  1010-5182     ISO Abbreviation:  J Craniomaxillofac Surg     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-31     Completed Date:  2007-01-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8704309     Medline TA:  J Craniomaxillofac Surg     Country:  Scotland    
Other Details:
Languages:  eng     Pagination:  387-94     Citation Subset:  D; IM    
Cattedra di Chirurgia Speciale Odontostomatologica, University of Pisa, Pisa, Italy.
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MeSH Terms
Age Factors
Aging / physiology*
Alkaline Phosphatase / analysis
Bone Morphogenetic Protein 6
Bone Morphogenetic Proteins / pharmacology
Cell Count
Cell Differentiation / drug effects,  physiology
Cell Movement / drug effects,  physiology
Cell Proliferation / drug effects
Cells, Cultured
Chemotactic Factors / pharmacology
Culture Media, Conditioned
Culture Media, Serum-Free
Mandible / cytology*,  drug effects
Mitogens / pharmacology
Platelet-Derived Growth Factor / pharmacology
Radiopharmaceuticals / diagnostic use
Rats, Wistar
Skull / cytology*,  drug effects
Transforming Growth Factor beta / pharmacology
Tritium / diagnostic use
Reg. No./Substance:
0/Bmp6 protein, rat; 0/Bone Morphogenetic Protein 6; 0/Bone Morphogenetic Proteins; 0/Chemotactic Factors; 0/Culture Media, Conditioned; 0/Culture Media, Serum-Free; 0/Mitogens; 0/Platelet-Derived Growth Factor; 0/Radiopharmaceuticals; 0/Transforming Growth Factor beta; 10028-17-8/Tritium; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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