Document Detail


Age and growth-related changes in cyclopiazonic acid-potentiated lipophilic cation accumulation by cultured cells and binding to freeze-thaw lysed cells.
MedLine Citation:
PMID:  3508477     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a previous study (1) we demonstrated that increased tetraphenylphosphonium (TPP) uptake by renal epithelial cells (LLC-PK1) exposed to the fungal metabolite cyclopiazonic acid (CPA) was not a result of hyperpolarization across the plasma membrane even though CPA-potentiated TPP uptake could be totally inhibited by the depolarizing agent carbonylcyanide-m-chlorophenylhydrazone (CCCP). We now demonstrate that CPA potentiates TPP accumulation by proliferating skeletal muscle (L6) and LLC-PK1 cells but not by nonproliferating primary rat hepatocytes. In LLC-PK1 cells, CPA-potentiated TPP accumulation is observed in cells at all ages. In L6 cells, CPA-potentiated TPP accumulation is maximal soon after subculturing, and as the cells age they become less sensitive to CPA until TPP accumulation by CPA-treated cells approaches that of untreated cells. The temporal change in sensitivity of L6 cells to CPA may be related to biochemical and/or metabolic changes which occur as the cells age in culture. Hepatocytes, LLC-PK1 cells, and L6 cells permeabilized by freeze-thaw lysis, all exhibit CPA-potentiated TPP partitioning, even in the presence of CCCP. This result indicates that both TPP and CPA must have access to the intracellular space in order for potentiated TPP partitioning to be observed. We hypothesize that the site of interaction between CPA and TPP is intracellular and probably associated with the cytoplasmic side of the plasma membrane and possibly the mitochondria.
Authors:
R T Riley; D E Goeger; W P Norred; R J Cole; J W Dorner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemical toxicology     Volume:  2     ISSN:  0887-2082     ISO Abbreviation:  J. Biochem. Toxicol.     Publication Date:    1987 Fall-Winter
Date Detail:
Created Date:  1989-06-09     Completed Date:  1989-06-09     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8700114     Medline TA:  J Biochem Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  251-64     Citation Subset:  IM    
Affiliation:
U.S. Department of Agriculture, Athens, GA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival
Cells, Cultured
Epithelium / metabolism
Freezing
Indoles / pharmacology*
Kidney / metabolism*
Liver / metabolism
Muscles / metabolism
Mycotoxins / pharmacology*
Onium Compounds / pharmacokinetics*
Organophosphorus Compounds / pharmacokinetics*
Phospholipids / isolation & purification
Rats
Time Factors
Chemical
Reg. No./Substance:
0/Indoles; 0/Mycotoxins; 0/Onium Compounds; 0/Organophosphorus Compounds; 0/Phospholipids; 18172-33-3/cyclopiazonic acid; 18198-39-5/tetraphenylphosphonium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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