| Age and gender effects on apoptosis in the human coronary arterial wall. | |
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MedLine Citation:
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PMID: 15888322 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The relationship between aging and apoptosis remains unclear. We wondered whether apoptosis could be enhanced in arterial aging in the absence of overt or advanced arterial disease. Apoptosis-related proteins were investigated using three methods: TdT-mediated dUTP digoxigenin nick end labeling (TUNEL) technique, active cysteine-dependant aspartate specific proteases (caspase)-3 and poly ADP-ribose polymerase (PARP) in coronary arteries of human subjects ranging from 25 to 92 years. We found no significant correlation between age and the apoptotic index using the three methods. The percentage of active caspase-3 positive cells was found to be significantly higher in men than in women (9.11 +/- 12.3 cells/mm(2) versus 2.01 +/- 4.55 cells/mm(2), respectively, p = 0.017). These sex-related differences did not reach statistical significance using TUNEL (9.93 +/- 17 and 2.61 +/- 4.58 cells/mm(2), p = 0.32) and PARP methods (3.42 +/- 7.74 and 0.86 +/- 0.95 cells/mm(2), p < 0.49). This is the first report of detection of apoptotic cells in the human arterial wall in adult subjects free from arterial diseases. Apoptosis is an attractive hypothesis to account for organ aging, but our study suggests that apoptosis is not a key factor in aging of the arterial wall. |
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Authors:
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Jacques Boddaert; Ziad Mallat; Paul Fornes; Bruno Esposito; Dominique Lecomte; Marc Verny; Alain Tedgui; Joël Belmin |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Mechanisms of ageing and development Volume: 126 ISSN: 0047-6374 ISO Abbreviation: Mech. Ageing Dev. Publication Date: 2005 Jun-Jul |
Date Detail:
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Created Date: 2005-05-12 Completed Date: 2005-09-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0347227 Medline TA: Mech Ageing Dev Country: Ireland |
Other Details:
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Languages: eng Pagination: 678-84 Citation Subset: IM |
Affiliation:
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INSERM U-541, IFR Circulation, Hôpital Lariboisière, Paris, France. jacques.boddaert@psl.ap-hop-paris.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Aging / metabolism*, pathology Apoptosis* Coronary Vessels / pathology, physiopathology* Female Humans Male Middle Aged Tunica Intima / pathology, physiopathology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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