Document Detail


Age and gender affect DNMT3a and DNMT3b expression in human liver.
MedLine Citation:
PMID:  17929180     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) including the maintenance enzyme DNMT 1 and de novo methyltransferases DNMT 3a and DNMT 3b. Elevated levels of DNMTs have been found in cancer cells and in several types of human tumors. A polymorphism found in DNMT3b has been associated with increased risk for several cancers. The factors influencing DNMT expression in human tissues have not been clearly determined. he present study examined TDNMT3a and DNMT3b levels in human liver tissue samples and compared the effect of ageing, cigarette smoking, and gender. DNMT3a and DNMT3b expression levels in the samples from older individuals (56-78 years, n = 28) were both significantly higher than those of the younger group (16-48 years, n = 27) (73.2 +/- 3.4 vs 8.3 +/- 2.8 and 56.1 +/- 1.9 vs 17.5 +/- 5.7, respectively; p < 0.05). Levels of DNMT3b in females were significantly higher than those in males (75.4 +/- 2.2 vs 16.3 +/- 4.7; p < 0.05); however, DNMT3a levels were similar for females and males (52.7 +/- 2.7 vs 48.4 +/- 2.0). Expression levels of DNMT3a and DNMT3b were similar in smokers and nonsmokers (58.1 +/- 3.5 vs 60.8 +/- 3.1 and 54.5 +/- 2.3 vs 48.3 +/- 1.8, respectively). Genotyping for DNMT3b (C-->T) variant in this sample pool showed a frequency distribution of CC (41%), CT (50%), and TT (9%). The findings from this study suggest that ageing and gender may be important factors influencing DNA methylation status.
Authors:
Yongmei Xiao; Beverly Word; Atena Starlard-Davenport; Aaron Haefele; Beverly D Lyn-Cook; George Hammons
Publication Detail:
Type:  Journal Article     Date:  2007-10-11
Journal Detail:
Title:  Cell biology and toxicology     Volume:  24     ISSN:  0742-2091     ISO Abbreviation:  Cell Biol. Toxicol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-04-22     Completed Date:  2008-08-05     Revised Date:  2010-05-21    
Medline Journal Info:
Nlm Unique ID:  8506639     Medline TA:  Cell Biol Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  265-72     Citation Subset:  IM    
Affiliation:
Division of Personalized Nutrition and Medicine, National Center for Toxicological Research, Jefferson, AR 72079, USA.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Age Factors*
Aged
Base Sequence
DNA (Cytosine-5-)-Methyltransferase / metabolism*
DNA Primers
Female
Humans
Liver / enzymology*
Male
Middle Aged
Sex Factors*
Chemical
Reg. No./Substance:
0/DNA Primers; EC 2.1.1.37/DNA (Cytosine-5-)-Methyltransferase; EC 2.1.1.37/DNA methyltransferase 3A; EC 2.1.1.37/DNA methyltransferase 3B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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