| Age-dependent thickening of glomerular basement membrane has no major effect on glomerular hydraulic conductivity. | |
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MedLine Citation:
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PMID: 15031333 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The effect of the increasing thickness of the glomerular basement membrane (GBM), which is seen in ageing rats, on the effective hydraulic conductivity (k) of the glomerular capillary wall was studied in Wistar rats aged 2 and 18 months. METHODS: With the use of micropuncture techniques, ultrafiltration characteristics of cortical glomeruli were determined in isolated cell-free perfused kidneys. Because the filtration fraction in this preparation is low (3%) as a consequence of high perfusion rates at glomerular filtration rates comparable with in vivo conditions, uniform ultrafiltration conditions are provided over the whole filtering surface. After fixation at a defined perfusion pressure, the surface of glomerular capillaries (S) was obtained morphometrically on light microscopic sections of the glomeruli studied previously. RESULTS: The glomerular ultrafiltration coefficient (K(f)) was 0.025 nl/s.mmHg in young rats and 0.038 nl/s.mmHg in old rats (P<0.0005) and S was 0.140 mm(2) in young and 0.244 mm(2) in old rats (P<0.0005). However, k was not significantly different (18.0 nl/s.mmHg.cm(2) in young and 15.8 nl/s.mmHg.cm(2) in old rats) despite a 2.4-fold increase of GBM thickness as estimated from electron microscopic sections. CONCLUSIONS: These findings indicate that the age-dependent increase of GBM thickness in rat kidneys did not substantially increase hydraulic resistance of the glomerular capillary wall. |
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Authors:
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Klaus H Neumann; Christian Kellner; Karlwilhelm Kühn; Hilmar Stolte; Hans-Joachim Schurek |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association Volume: 19 ISSN: 0931-0509 ISO Abbreviation: Nephrol. Dial. Transplant. Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-03-19 Completed Date: 2004-10-07 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8706402 Medline TA: Nephrol Dial Transplant Country: England |
Other Details:
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Languages: eng Pagination: 805-11 Citation Subset: IM |
Affiliation:
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Klinik für Nephrologie, Universitätsklinikum, Leipziger Strasse 44, D-39120 Magdeburg, Germany. Klaus.Neumann@medizin.uni-magdeburg.de |
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| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Animals Basement Membrane / pathology Biophysical Phenomena Biophysics Kidney Glomerulus / anatomy & histology, blood supply, pathology*, physiology* Male Microcirculation Perfusion Rats Rats, Wistar |
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