Document Detail


Age-dependent sensitivity of the developing brain to irradiation is correlated with the number and vulnerability of progenitor cells.
MedLine Citation:
PMID:  15659227     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a newly established model of unilateral, irradiation (IR)-induced injury we compared the outcome after IR to the immature and juvenile brain, using rats at postnatal days 9 or 23, respectively. We demonstrate that (i) the immature brains contained more progenitors in the subventricular zone (SVZ) and subgranular zone (SGZ) compared with the juvenile brains; (ii) cellular injury, as judged by activation of caspase 3 and p53, as well as nitrotyrosine formation, was more pronounced in the SVZ and SGZ in the immature brains 6 h after IR; (iii) the number of progenitor and immature cells in the SVZ and SGZ decreased 6 h and 7 days post-IR, corresponding to acute and subacute effects in humans, respectively, these effects were more pronounced in immature brains; (iv) myelination was impaired after IR at both ages, and much more pronounced after IR to immature brains; (v) the IR-induced changes remained significant for at least 10 weeks, corresponding to late effects in humans, and were most pronounced after IR to immature brains. It appears that IR induces both an acute loss of progenitors through apoptosis and a perturbed microenvironment incompatible with normal proliferation and differentiation, and that this is more pronounced in the immature brain.
Authors:
Aya Fukuda; Hirotsugu Fukuda; Janos Swanpalmer; Sven Hertzman; Birgitta Lannering; Ildiko Marky; Thomas Björk-Eriksson; Klas Blomgren
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  92     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-20     Completed Date:  2005-06-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  569-84     Citation Subset:  IM    
Affiliation:
Arvid Carlsson Institute for Neuroscience, Department of Clinical Neuroscience, Sahlgrenska Academy, Göteborg University, SE-405 30 Göteborg, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Antigens, Differentiation / biosynthesis
Brain / growth & development*,  pathology,  radiation effects*
Caspase 3
Caspases / metabolism
Cell Count
Dentate Gyrus / growth & development,  pathology,  radiation effects
Disease Models, Animal
Female
Functional Laterality / radiation effects
Lateral Ventricles / growth & development,  pathology,  radiation effects
Male
Myelin Sheath / metabolism,  pathology
Particle Accelerators
Radiation Injuries, Experimental / metabolism*,  pathology
Radiation Tolerance / physiology*
Rats
Rats, Wistar
Stem Cells / pathology,  radiation effects*
Tumor Suppressor Protein p53 / metabolism
Tyrosine / analogs & derivatives*,  metabolism
Chemical
Reg. No./Substance:
0/Antigens, Differentiation; 0/Tumor Suppressor Protein p53; 3604-79-3/3-nitrotyrosine; 55520-40-6/Tyrosine; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases
Comments/Corrections
Erratum In:
J Neurochem. 2005 Dec;95(6):1802

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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